抑制性受体LAIR-1对巨核谱系分化的调节作用  

The regulation of inhibitory receptor LAIR-1 on the differentiation of megakaryocytic lineage

作　　者：张晓姝[1] 赵海亚[1] 冯小英[1] 曹晏宁[1] 付爱丽[1] 薛江楠[1] 

机构地区：[1]滨州医学院免疫学教研室,山东烟台264003

出　　处：《基础医学与临床》2012年第3期261-267,共7页Basic and Clinical Medicine

基　　金：国家自然科学基金(81001300);山东省自然科学基金(Y2007C009);山东省优秀中青年科学家科研奖励基金(2008BS03013)

摘　　要：目的研究抑制性受体LAIR-1在巨核谱系细胞的表达及其对巨核细胞分化的调节作用。方法采用流式细胞术和激光共聚焦显微镜检测LAIR-1在巨核谱系细胞的表达;免疫磁珠法纯化脐血CD34+细胞,体外无血清培养系统诱导CD34+细胞向巨核细胞分化,观察LAIR-1被抗体或配体交联激活后对巨核细胞分化的影响。结果 LAIR-1表达于人骨髓CD34+CD41a+和CD41a+CD42b+细胞以及脐血CD34+CD41a-和CD34+CD41a+细胞;脐血CD34+细胞向巨核细胞分化过程中,LAIR-1表达在倍性为2N和4N的不成熟CD41a+细胞上;交联激活LAIR-1分子能够抑制脐血CD34+细胞向巨核细胞的分化。结论 LAIR-1可能是参与巨核细胞早期分化发育的一种新的负调控分子。Objective To study the expression of inhibitory receptor LAIR-1 on the megakaryocytic lineage cells and the regulatory effect of LAIR-1 on the differentiation of megakaryocytes（MKs）.Methods Flow cytometry（FCM） and laser confocal microscopy were used to detect the expression of LAIR-1 on the megakaryocytic lineage cells.Cord blood CD34＋ cells were purified by a positive selection using an immunomagnetic separation system.The CD34＋ cells were induced to differentiate to MKs by serum-free medium system in vitro.LAIR-1 was activated by cross-linking with antibody or ligand and then the effect of LAIR-1 on the differentiation of MKs was detected.Results LAIR-1 was expressed on the surface of CD34＋CD41a＋ and CD41a＋CD42b＋ cells in the bone marrow of human.In human cord blood,the surface expression of LAIR-1 is detectable in CD34＋CD41a-and CD34＋CD41a＋ cells.When cord blood CD34＋ cells were induced to megakaryocytic differentiation,LAIR-1 presented in immature CD41a＋ cells with cell ploidy of 2N and 4N.Cross-linking of LAIR-1 appeared to inhibit the differentiation of MKs from cord blood CD34＋ cells.Conclusions LAIR-1 is a new potential negative regulatory molecule participating in the early-stage differentiation of MKs.

关 键 词：LAIR-1 巨核细胞 骨髓 脐血 

分 类 号：R392.1[医药卫生—免疫学]