包裹紫杉醇的高分子纳米粒与超声脂质微泡复合体的制备研究  

Production of the Complex of Paclitaxel Loaded PLGA-COOH Nanoparticles and Ultrasound Microbubbes

作　　者：容跃[1] 冉海涛[1] 王志刚[1] 王红红[1] 宫玉萍[1] 刘利[1] 

机构地区：[1]重庆医科大学附属第二医院超声影像学研究所,重庆市400010

出　　处：《中国超声医学杂志》2012年第2期97-100,共4页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金面上项目(No.30770565);重庆市科技攻关项目(No.2009AB5216)

摘　　要：目的制备一种利用生物素亲和素技术耦联的新型包裹紫杉醇的微泡,并对其连接效果、理化性质进行检测。方法采用单乳化法制备包裹紫杉醇的PLGA-COOH纳米粒(Pac PLGA),检测其包封率,并用碳二亚胺法将其与链霉亲和素(SA)耦联,免疫荧光法检测Pac-PLGA与SA的连接情况。采用机械振荡法制备生物素化的超声微泡(BIOOMB)及超声微泡(MB)。分2组用激光共聚焦不同时间点观察超声微泡与Pac PLGA的连接效果。结果 Pac PLGA平均粒径为131.1 nm,包封率为(85±2.1)%,电镜观察呈球形,大小均匀,分散度好。荧光检测SA成功的连接在Pac-PLGA上。激光共聚焦观察生物素-亲和素组Pac-PLGA较多地连接在BIO-MB的表面。而对照组MB表面未见Pac PLGA的聚集。结论本实验成功地制备出包裹紫杉醇的新型载药微泡,有望为乳腺癌治疗提供一种理想的药物载体和靶向给药系统。Objective To prepare a use of adivin-biotin technique coupled new Paelitaxe-loaded microbubble and test its effect and physical and chemical properties. Methods The Paclitaxe loaded high molecular polymer PLGA- COOH nanopartieles（Pac-PLGA） produced by the single emulsion technique integrated with streptoavidin were pro- duced using EDC/NHS. then detect the encapsulation efficiency. The connection between the Pac-PLGA and streptoa- vidin was detected by immunofluorescence. MB-NH2 and MB were prepared by mechanical shaking. The study was di- vided into two groups. The connection effect was observed under laser confoca[ microscope at different time. Results The average diameter of Pac-PLGA was 131.1 nm. The encapsulation efficiency was （85-4-2.1）~. It had uniform size, good dispersion under the electron microscopy. Immunofluorescent assay showed that streptoavidin was successfully conjugated on the surface of Pac-PLGA. In contral group （MBA-NP-COOH）, Pae-PLGA were not conjugated to the surface of microbubbles. However, in adivin-biotin group（BIO-MBq-SA-NP-COOH）, it showed that a number of Pae-PLGA gathered around microbubbles like a garland under laser confocal microscope. Conclusions A noval ultra- sound mierobubble-drug are prepared successfully. It is potential to provide a new drug delivery and targeted drug de- livery system on tumor therapy.

关 键 词：紫杉醇 PLGA纳米粒 超声微泡 

分 类 号：R943[医药卫生—药剂学]