重组L-门冬酰胺酶Ⅱ的提取纯化工艺  

Study on Extraction and Purification Technique of Recombinant L AsparaginaseⅡ

作　　者：吴敬[1] 赖龙生[1] 刘景晶[1] 吴梧桐[1] 

机构地区：[1]中国药科大学生化教研室,江苏南京210009

出　　处：《中国医药工业杂志》2000年第2期50-53,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"九五"科技攻关项目! (编号 96 90 2 0 1)

摘　　要：采用渗透振扰破壁法、硫酸铵分级沉淀、乙醇分级沉淀、DEAE-纤维素层析等步骤提取和纯化重组L-门冬酰胺酶,并优化了提纯过程的条件,提取总回收率高于50%,产品纯度经高效毛细管电泳检测为单一峰,具有工业生产前景。A combined technique of permeation vibration, (NH 4) 2SO 4 fractionation, ethanol fractionation and DEAE cellulose chromatography was used to extract and purify the recombinant L asparaginase Ⅱ.The total recovery of enzyme was greater than 50% by optimizing these procedures.HPCE analysis gave a final product of a single peak.

关 键 词：L-门冬酰胺酶Ⅱ 分离 纯化 

分 类 号：R979.1[医药卫生—药品] Q556.403[医药卫生—药学]