磷酸肌酸钠对缺氧幼鼠脑组织中钙调蛋白、一氧化氮水平的影响  

Effects of creatine phosphate sodium on levels of CaM,NO in brain tissue of anoxia young rats

作　　者：桂静[1] 陈莹[1] 李永凤[1] 刘春艳[1] 

机构地区：[1]郑州大学第二附属医院儿科,郑州450014

出　　处：《郑州大学学报（医学版）》2012年第1期63-66,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究磷酸肌酸钠(PCr)对缺氧幼鼠脑组织中钙调蛋白(CaM)及一氧化氮(NO)水平的影响。方法:将75只SD幼鼠随机分为磷酸肌酸钠治疗组(PCr组)、模型组和正常对照组3组,PCr组和模型组制成缺氧模型,并于缺氧前0.5h、缺氧后即刻、24h和48h分别腹腔注射PCr和等量生理盐水,正常对照组不作缺氧处理。各组大鼠于缺氧后6h观察脑组织的病理改变,于48h检测脑组织中CaM及NO含量的变化。结果:缺氧后6h,PCr组较模型组神经细胞破坏减少,水肿、坏死明显减轻。3组幼鼠脑组织中CaM和NO含量差异均有统计学意义(F分别为27.425和22.137,P<0.001)。PCr组的CaM含量[(41.19±1.13)ng/L]较正常对照组[(39.56±0.86)ng/L]稍高(P<0.05),但较模型组[(42.04±0.76)ng/L]降低(P<0.05);PCr组的NO含量[(0.73±0.13)μmol/g]较正常对照组[(0.55±0.07)μmol/g]稍高(P<0.05),但较模型组[(0.86±0.17)μmol/g]降低(P<0.05)。结论:PCr可以通过降低脑组织中CaM、NO的含量而发挥对缺氧所致的脑损伤的神经保护作用。Aim：To observe the influence of creatine phosphate sodium（PCr） on the level of calmodulin（CaM）and nitric oxide（NO） in anoxia young rats＇ brain tissue.Methods：A total of 75 young rats were randomly divided into three groups,PCr group,model group and normal control group,25 for each group.The rats of PCr group and model group were established anoxia model,and intraperitoneally injected PCr and equal NS respectively.The normal control group was not treated.On anoxic 6 h the pathological changes of the brain was observed,on 48 h the CaM and NO content changes was observed.Results：The contents of CaM and NO in brain tissue of three groups had significant difference（F=27.425,22.137,P0.001）.After anoxia,compared with the model group,neural cell destruction of PCr group reduced,and edema,necrosis eased significantly;the CaM content of PCr group [（41.19±1.13） ng/L ] was a little bit higher（P〈0.05） than normal control group（39.56±0.86） ng/L,but lower（P〈0.05） than model group [（42.04±0.76） ng/L ].The No content of PCr group [（0.73±0.13） μmoL/g] was higher than that of normal control group [（0.55±0.07） μmoL/g],but lower than that of model group [（0.86±0.17） μmoL/g].Conclusion：PCr may have neural protection by means of decreasing the CaM and NO content during brain injury by anoxia.

关 键 词：磷酸肌酸钠 缺氧 钙调蛋白 一氧化氮 大鼠 脑 

分 类 号：R722.1[医药卫生—儿科]