膈下逐瘀汤对大鼠纤维化肝组织α-SMA和TGF-β1表达的影响  

Effects of Gexia Zhuyu Tang on expressions of α-SMA and TGF-β1 in fibrous liver tissues of rats

作　　者：杨婧[1] 贾彦[1] 刘宏[1] 代巧妹[1] 王蔚[1] 王亚贤[1] 冯涛[1] 华东[1] 肖佳音[1] 

机构地区：[1]黑龙江中医药大学基础医学院病理教研室,哈尔滨150040

出　　处：《中国中医基础医学杂志》2012年第2期158-160,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：黑龙江省自然科学基金资助项目(D200820);黑龙江省教育厅科学技术研究面上项目(11541344);黑龙江省中医药科研指导项目(ZHY08-W04)

摘　　要：目的:观察膈下逐瘀汤抗猪血清诱导的大鼠肝纤维化作用及其可能的作用机制。方法:采用猪血清腹腔注射诱导大鼠免疫性肝纤维化模型,VG染色法检测肝组织病理学改变,ELISA检测肝组织TGF-β1含量,定量RT-PCR检测肝组织TGF-β1和α-SMA mRNA表达。结果:与模型组大鼠比较,膈下逐瘀汤可显著降低猪血清诱导的肝纤维化程度,减少肝组织TGF-β1蛋白和mRNA表达,降低肝组织α-SMA mRNA表达。结论:膈下逐瘀汤可有效地逆转实验性大鼠肝纤维化,其机制可能与抑制肝星状细胞活化有关。Objective： To investigate the inhibitory effect and possible mechanism of Gexiazhuyu decoction on hepatic fibrosis induced by porcine serum in rats.Methods： Sixty male Sprague-Dawley rats were randomly divided into four groups as follows： blank control group,Gexiazhuyu decoction group,porcine serum group,porcine serum plus Gexiazhuyu decoction group.The fibrosis model of rats was achieved by intraperitoneal injection of porcine serum twice weekly for 12 weeks,simultaneously Gexiazhuyu decoction administered daily by gavage for 12 weeks.All rats were sacrificed at the end of experiment,and liver were collected for analysis.The liver histopathology was examined by van Gieson＇s stain,and the content of TGF-β1 in the liver were detected by enzyme linked immunosorbent assay.Real-time reverse transcriptase polymerase chain reaction was used to analyze α-SMA and TGF-β1 mRNA expression in the liver.Results： Compared with the porcine serum group,the histopathology of rats given Gexiazhuyu decoction improved significantly.α-SMA mRNA expressions were decreased significantly,and TGF-β1 mRNA expressions and content in liver were decreased significantly in porcine serum plus Gexiazhuyu decoction group（P 0.05）.Conclusions： Gexiazhuyu decoction may exert anti-hepatic fibrosis effect in rats via inhibition of HSC activation

关 键 词：肝纤维化 膈下逐瘀汤 转化生长因子-Β1 Α-平滑肌肌动蛋白 

分 类 号：R287.5[医药卫生—中药学]