HIV感染者CD_4^+调节性T细胞与HIV DNA呈正相关  

CD_4^+ regulatory T cells are positively correlated with HIV DNA in HIV infected individuals

作　　者：郭彩萍[1] 画伟[1] 计云霞[1] 张彤[1] 陈德喜[1] 吴昊[1] 焦艳梅[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染中心,北京100069

出　　处：《中国艾滋病性病》2012年第1期10-13,共4页Chinese Journal of Aids & STD

基　　金：国家"十一五"传染病重大专项(2008ZX10001-006);北京市科委资助项目(D0906003040391);国家自然基金项目(30872226)~~

摘　　要：目的分析CD4+调节性T细胞与艾滋病病毒(HIV)脱氧核糖核酸(DNA)之间的关系。方法随机选择门诊病人50例,采用流式细胞仪细胞内染色技术检测CD4+CD2+5Foxp3+调节性T细胞的水平,并用实时定量聚合酶链反应(PCR)检测外周血单个核细胞中的DNA水平,分析CD4+调节性T细胞与CD4计数、病毒载量及HIV-1DNA之间的相关性。结果在HIV感染病人CD4+调节性T细胞与HIV DNA呈正相关,与CD4计数呈正相关,与病毒载量呈负相关。结论未进行治疗的HIV病人,其CD4+调节性T细胞与HIV DNA呈正相关,说明CD4+调节性T细胞越多,HIV-1DNA越多,CD4+调节性T细胞可能对机体发挥不利的作用。Objective To analyze the relationship between CD＋4 regulatory T cells and HIV DNA.Methods Fifty outpatient visitors were randomly selected in Youan Hospital.Intracellular staining and flow cytometry were used to detect CD＋4 CD＋25 Foxp＋3 regulatory T cell level,and real-time PCR quantitative method was used to detect peripheral blood mononuclear cells DNA levels.The correlation between CD＋4 regulatory T cells,CD4 count,viral load and HIV-1DNA was analyzed.Result In HIV infected individuals there was positive correlation between CD＋4 regulatory T cells and HIV DNA and CD4 count,but negative correlation between CD＋4 regulatory T and viral load.Conclusion In the naive HIV individuals who had not received treatment,there was positive correlation between CD＋4 regulatory T cells and HIVDNA,indicating that more CD＋4 regulatory T cells,and more HIV-1DNA were present.Therefore,CD＋4 regulatory T cells might play a negative role to the human body.

关 键 词：调节性T细胞 艾滋病病毒感染者 脱氧核糖核酸 

分 类 号：R512.91[医药卫生—内科学]