All-optical Quantitative Framework for Bioluminescence Tomography with Non-contact Measurement  

作　　者：Xue-Li Chen Heng Zhao Xiao-Chao Qu Duo-Fang Chen Xiao-Rui Wang Ji-Min Liang 

机构地区：[1]Life Sciences Research Center, School of Life Sciences and Technology, Xidian University, Xi＇an 710071, PRC [2]School of Technical Physics, Xidian University, Xi＇an 710071, PRC

出　　处：《International Journal of Automation and computing》2012年第1期72-80,共9页国际自动化与计算杂志（英文版）

基　　金：supported by National Basic Research Program of China (973 Program) (No.2011CB707702);National Natural Science Foundation of China (No.81090272, No.81000632, and No.30900334);Shaanxi Provincial Natural Science Foundation Research Project (No.2009JQ8018);Fundamental Research Funds for the Central Universities

摘　　要：In this contribution, we present an all-optical quantitative framework for bioluminescence tomography with non-contact measurement. The framework is comprised of four indispensable steps： extraction of the geometrical structures of the subject, light flux reconstruction on arbitrary surface, calibration and quantification of the surface light flux and internal bioluminescence reconstruction. In particular, the geometrical structures are retrieved using a completely optical method and captured under identical viewing conditions with the bioluminescent images. As a result, the proposed framework avoids the utilization of computed tomography or magnetic resonance imaging to provide the geometrical structures. On the basis of experimental measurements, we evaluate the performance of the proposed all-optical quantitative framework using a mouse shaped phantom. Preliminary result reveals the potential and feasibility of the proposed framework for bioluminescence tomography.In this contribution, we present an all-optical quantitative framework for bioluminescence tomography with non-contact measurement. The framework is comprised of four indispensable steps： extraction of the geometrical structures of the subject, light flux reconstruction on arbitrary surface, calibration and quantification of the surface light flux and internal bioluminescence reconstruction. In particular, the geometrical structures are retrieved using a completely optical method and captured under identical viewing conditions with the bioluminescent images. As a result, the proposed framework avoids the utilization of computed tomography or magnetic resonance imaging to provide the geometrical structures. On the basis of experimental measurements, we evaluate the performance of the proposed all-optical quantitative framework using a mouse shaped phantom. Preliminary result reveals the potential and feasibility of the proposed framework for bioluminescence tomography.

关 键 词：ALL-OPTICAL biol tomography quantitative framework non-contact surface extraction. 

分 类 号：O482.31[理学—固体物理] TG84[理学—物理]