阴道毛滴虫病毒对虫体基因多态性的影响  

The effects of the Trichomonas vaginalis virus on genetic polymorphisms of Trichomonas vaginalis

作　　者：王丽娟[1] 王黎[1] 薛长贵[1] 

机构地区：[1]郑州大学基础医学院寄生虫学教研室,河南郑州450001

出　　处：《中国病原生物学杂志》2012年第1期34-36,7,共4页Journal of Pathogen Biology

基　　金：河南省医学科技攻关基金项目(No.200804004)

摘　　要：目的分析阴道毛滴虫病毒对阴道毛滴虫临床分离株基因多态性的影响。方法用MGE-PCR扩增阴道毛滴虫病毒阳性株和病毒阴性株Tvmar1基因位置,应用软件MEGA 5.0构建系统进化树。结果阴道毛滴虫临床分离株之间的遗传差异明显,6株阴道毛滴虫病毒阳性株之间的遗传距离较近,分布在同一分支上。结论阴道毛滴虫病毒会对阴道毛滴虫的基因多态性产生影响。Objective To analyze the effects of Trichomonas vaginalis virus on genetic polymorphisms of T.vaginalis. Methods Thirty T.vaginalis isolates were subjected to MGE-PCR to detect variations in the location of the Tvmar1 gene.Genetic polymorphisms due to the presence of TVV were analyzed and based on the results phylogenetic trees were constructed using MEGA 5.0.Results The variable location of Tvmar1 genes from 30 T.vaginalis isolates showed that the protozoan isolates have significant genetic polymorphism.Based on the results of MGE-PCR,the phylogenetic tree of Tvmar1 genes indicated that the 6 T.vaginalis virus-positive isolates were in the same cluster.Conclusion The presence of T.vaginalis virus affects the genetic polymorphisms of parasites.

关 键 词：阴道毛滴虫 阴道毛滴虫病毒 MGE-PCR 基因多态性 

分 类 号：R382.21[医药卫生—医学寄生虫学]