3种旋毛虫成虫永久标本染色方法的比较  

A comparative study of three methods of staining to prepare permanent specimens of adult Trichinella spiralis worms

作　　者：杨丁[1] 皮本伟[1] 李丹[1] 牛利娜[1] 张莹[1] 王国英[2] 赵粤萍[2] 

机构地区：[1]河南大学医学院临床医学2009级,河南开封475004 [2]河南大学医学院免疫学研究所

出　　处：《中国病原生物学杂志》2012年第1期46-47,共2页Journal of Pathogen Biology

基　　金：河南大学大学生创新性实验资助课题(No.2010-10NB032)

摘　　要：目的探讨制作旋毛虫成虫染色标本的最佳方法。方法分别使用乙醇硼砂卡红、醋酸卡红、固绿3种染色法制作旋毛虫成虫标本。结果乙醇硼砂卡红染色的标本,颜色鲜艳,结构清晰,另外两种染液染色效果不佳。结论乙醇硼砂卡红染色可用于旋毛虫成虫永久标本的制作。Objective To determine the best method of staining to prepare permanent specimens of adult Trichinella spiralis worms.Methods Alcohol-borax-carmine,acetic acid carmine,and fast green were each used to prepare specimens of adult T.spiralis worms.Results Specimens stained with alcohol-borax-carmine were brighter and had a more readily apparent internal structure than specimens stained by other methods.Conclusion Alcohol-borax-carmine staining can be used to prepare permanent specimens of adult T.spiralis worms.

关 键 词：旋毛虫 成虫 标本制作 

分 类 号：R383.15[医药卫生—医学寄生虫学]