新疆维吾尔族人类白细胞抗原-DRB1基因多态性与乙型肝炎病毒感染结局  

Association of HLA-DRBI polymorphism with the outcomes of hepatitis B virus infection in Uygur population of Xinjiang

作　　者：杨志[1] 杨晓筠[1] 张永萍[1] 努斯来提[1] 仲英娜[1] 

机构地区：[1]新疆维吾尔自治区人民医院感染科,乌鲁木齐830001

出　　处：《国际流行病学传染病学杂志》2012年第1期20-24,共5页International Journal of Epidemiology and Infectious Disease

基　　金：新疆维吾尔自治区人民医院院内科研项目（20100105）

摘　　要：目的探讨新疆维吾尔族人群HLA—DRB1等位基因多态性与HBV感染临床转归及HBV不同复制状态的相关性。方法采用序列特异性引物-PCR方法对70例慢性乙型肝炎患者（CHB组）、4J6例HBV携带者（携带组）、42例HBV感染后自然恢复者（恢复组）、80名健康献血员（对照组）进行HJA-DRB1等位基因分型比较，并比较HBV不同复制状态下DRB1多态性的分布频率及其差异。结果HBV感染后恢复组与对照组HLA-DRB1＊0701等位基因频率分别为13．09％和13．75％，均分别高于CHB组（5．71％）及携带组（4．35％），差异有统计学意义（70：4．06、6．03，P〈0．05，OR=0．36、0．34；72=4．75、6．31，P〈0．05，OR=0．27、0．25）；恢复组DRB1＊1201—3等位基因频率16．67％，高于CHB组（7．86％），差异有统计学意义（70=4．70，P〈0．05，OR=0．37）；DRB1＊1301—2基因在对照组和恢复组的频率分别9．38％、10．70％，显著高于CHB组，差异有统计学意义（y。=14．58、13．54，P〈0．01）；其他各等位基因频率在各组间差异无统计学意义；HBV不同复制状态H1A—DRB1等位基因分布频率差异无统计学意义（P〉0．05）。结论新疆维吾尔族携带HLA-DRB1＊0701、DRB1＊1201—3、DRB1＊1301—2基因型可能降低慢性乙型肝炎发生的风险，是HBV感染的抗性基因。Objective To investigate the associations of human leukocyte antigen （HLA）-DRB1 polymorphism with the outcome of HBV infection and the replication of HBV in Uygur population of Xinjiang. Methods Polymerase chain reaction-sequence specific primers （PCR-SSP） was used to genotype HLA-DRB1 gene. 70 chronic hepatitis B patients （CHB group）,46 chronic asymptomatic hepatitis B virus carriers（carrier groups）, 42 spontaneous rehabilitee after HBV infection（rehabilitee group） and 80 healthy controls were recruited. The frequency distributions of genotype among four groups were analyzed, and the frequency of that in different replication of HBV was also studied. Results The fiequency of HLA-DRB1 ＊ 0701 aUele in rehabifitee group（ 13.09% ） and control group （ 13.75% ） were significantly higher than that in CHB group（5.71%） χ2 = 4.06, 6.03, P 〈 0.05, OR = 0.36,0.34） and in carriers group （4.35%） （ χ2 = 4.75,6.31, P 〈 0.05, OR = 0.27,0.25）. Tbe frequency of HLA-DRB1 ＊ 1201-3 allele in rehabilitee group was higher than that in CHB group （16.67% ：7.86%, χ2 = 4.70, P 〈 0.05, OR = 0.37）. The frequencies of HLA- DRB1 ＊ 1301-2 in control group and rehabilitee group were 9.38 % and 10.7 %, significantly higher than that in CHB group（ χ2 = 13.54,14.58, P 〈 0.01 ）. The frequencies of other DRB1 alleles were no significant differences among the groups, and the same result was observed in different replication of HBV（ P 〉 0.05）. Conclusions Alleles of HLA-DRB1 ＆ 0701, DRB1 ＊ 1201-3 and DRB1 ＊ 1301-2 are closely aseciated with HBV clearance, which may be the resistant genes for CHB in Uygur population of Xinjiang.

关 键 词：肝炎病毒 乙型 HLA-DRB1 维吾尔族 

分 类 号：R51[医药卫生—内科学]