中药咳喘落对哮喘大鼠肺组织白细胞介素-4,5mRNA影响及相关性研究  

Study of effects of KeChuanLuo on the expressions of interleukin- 4,5 mRNA in lung tissues from asthmatic rat

作　　者：王宏长[1] 倪伟[2] 于素霞[1] 吴银根[1] 

机构地区：[1]上海中医药大学附属龙华医院呼吸内科,200032 [2]上海中草药大学附属龙华医院呼吸内科,200032

出　　处：《广东医学》2000年第2期107-109,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助!(编号:39670889)

摘　　要：目的探讨中药咳喘落拮抗支气管哮喘气道变应性炎症作用的分子机理。方法采用逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）法半定量地分析了哮喘大鼠肺组织 ＩＬ－４ ｍＲＮＡ和 ＩＬ－５ ｍＲＮＡ表达水平的变化及二者的相关性。结果哮喘时大鼠肺组织中 ＩＬ－４ ｍＲＮＡ与 ＩＬ－５ ｍＲＮＡ表达均增强，其相对表达量分别为 ０．４４２±０．０５２和０．６００±０．２２０，显著高于空白对照组（Ｐ＜０．０１），经大剂量咳喘落治疗后，其表达同时显著降低（Ｐ＜０．０１），治疗前后ＩＬ－４ｍＲＮＡ和ＩＬ－５ｍＲＮＡ表达水平分别呈显著正相关（ｒ＝０．７７２，０．８６８，Ｐ＜０．０１）。但小剂量咳喘落只对ＩＬ－４ｍＲＮＡ有下调作用（Ｐ＜０．０５）。结论咳喘落能够同时降低哮喘大鼠肺组织中ＩＬ－４和ＩＬ－５基因转录，减少其生物合成，进而抑制嗜酸性粒细胞的聚集、活化，发挥其抗气道炎症的作用。Objective To study the molecular mechanism of anti - airway allergic inflammation effects of traditional chinese herbs named KeChuanLuo. Methods The expression of interleukin - 4, 5(IL - 4,5)mRNA in lung tissues from asthmatic rat were examined semi - quantitively by reverse transcription- polymerase chain reaction(RT- PCR). Results The levels of IL- 4,5 mRNA expression in model group were higher than there in normal group(0.442 ± 0.052 and 0.600 ± 0.220 vs 0. 179 ± 0.037 and 0. 159 ± 0.052 respectively, P < 0.01 ), but expression levels was lower in higher dose KeChuanLuo -treat- ed group (P < 0.01). There were significantly positive correlations between the expression levels of IL- 4 mRNA and IL- 5 mRNA before and after treatment with higher dose of KeChuanLuo(r = 0.772, 0.868, P < 0.01). Only lower dose of KeChuanLuo inhibited the expression levels of IL- 4 mRNA. Conclusions The anti - airway allergic inflammation effects of KeChuanLuo are showed by marked inhibition of IL - 4,5 mRNA expression in lung tissues, reduced synthesis of IL - 4,5 and by conseqent depression of eosinophil accumulation and activation in the airway.

关 键 词：哮喘 咳喘落 白细胞介素 MRNA 中医药疗法 

分 类 号：R259.622.5[医药卫生—中西医结合]