耳针对兔急性实验性高血压的降压作用及机制的研究  

Antihypertensive Effects and Mechanism of Ear Acupuncture on Hypertension Rabbits

作　　者：木丽仙[1] 韩毅[1] 周文琪[1] 

机构地区：[1]昆明医学院生理教研室,云南昆明650031

出　　处：《昆明医学院学报》2012年第1期3-7,共5页Journal of Kunming Medical College

基　　金：国家自然科学基金资助项目(30160024)

摘　　要：目的观察耳针对兔急性实验性高血压的降压作用,并进一步探讨其可能的中枢机制.方法于股静脉匀速注射去甲肾上腺素(NE)制作急性实验性高血压模型,针刺耳廓"胸肺区"(TPA),观察是否有降压作用,采用中枢双侧旁巨细胞外侧核(PGL)微量注射阿片受体阻断剂纳络酮,探讨其可能的中枢机制.结果(1)股静脉匀速注射去甲肾上腺素后,可造成稳定的高血压状态;(2)双侧旁巨细胞外侧核内分别微量注射生理盐水(NS)、阿片受体阻断剂纳络酮(NAL),对急性实验性高血压无明显影响;(3)耳针TPA对急性实验性高血压具有显著的降压效应,mAP、mLVSP显著下降,HR有所下降.而PGL内微量注射纳络酮后耳针TPA能显著削弱耳针TPA引起的降压作用,使耳针后mAP、mLVSP及下降幅度显著减小,但不能完全阻断耳针TPA引起的降压作用.结论耳针兔耳廓"胸肺区"对急性实验性高血压具有降压效应,这种调整作用可能与包括PGL在内的头端延髓腹外侧区心血管中枢神经元内阿片肽的介导有密切关系.Objective To investigate the antihypertensive effects and mechanism of ear acupuncture on hypertension rabbits. Methods The hypertension model in rabbits was induced through injection of Norepinephrine （NE） into femoral vein. Naloxone was injected respectively into two side of Nucleus Paragigantocellularist Lateralis PGL, then ear acupuncture was performed on the hypertension rabbits, then we observed the changes of blood pressure and explored the possible mechanism. Results （1） Injection of Norepinephrine into femoral vein can induce stable acute breadboard hypertension. （2） When NS and NAL was microinjected respectively into PGL, there was no significant change in the blood pressure of hypertension rabbits. （3） The acupuncture of TPA had significant antihypertensive effects on hypertension rabbits, mAP and mLVSP decreased significantly and HR also detreased slightly, while microinjection of NAL into PGL counteracted but not blocked the function of acupuncture, decreased the extent of the variabilities of mAP, mLVSP and LV＋dp/dt max and HR. Conclusions Acupuncture of ＂thoracopneumatic area＂ can decrease the blood pressure of hypertension rabbits induced by injection of Norepinephrine （NE） into femoral vein. And this effect may partially rely on the releasing or activaction of Endogenous Opioid Peptide （Eop） in rostra] venteral lateral medulla cardiovascular center including PGL in medulla.

关 键 词：实验性高血压 耳针 耳廓“胸肺区” 旁巨细胞外侧核 内源性阿片肽 纳络酮 

分 类 号：R338.22[医药卫生—人体生理学]