钙拮抗剂硝苯吡啶对大鼠心肌缺血再灌注损伤的作用  

Protective Effect of Calcium Antagonist Nifedipine on Myocardial Ischemia-reperfusion Injury in Isolated Rat Hearts

作　　者：谷思洋[1,2] 邓立菊[2] 姜宏宇[1] 

机构地区：[1]吉林大学第一医院干部病房,吉林长春130021 [2]大庆龙南医院,黑龙江大庆163453

出　　处：《中国实验诊断学》2012年第2期210-213,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省自然科学基金资助(201115066)

摘　　要：目的研究钙拮抗剂(Calcium antagonists)硝苯吡啶(Nifedipine)对离体大鼠心肌缺血再灌注损伤的作用。方法 30只体重在260-300g的Wistar雄性大鼠随机分为三组:对照组、模型组、Nifedipine组(1μmmol/L)。大鼠麻醉后取出心脏,悬挂于Langendorff灌流装置上行主动脉逆行灌流,制备大鼠离体心脏缺血30min、再灌注120min模型;对照组行150min正常灌流。测定心肌梗死面积,检测SOD(Superoxidedismutas超氧化物歧化酶)及MDA(mal-onaldehyde丙二醛)的含量,免疫组化方法行PKCδ(The protein kinase C蛋白激酶C)的测定,用RT-PCR法测定NCX(Na+/Ca2+exchanger钠钙交换体)及SERCA2α(Sareoplasmie reticulum calcium adenodine triphosphatase肌浆网钙泵)的表达。结果硝苯吡啶组心肌梗死面积较模型组明显缩小(P<0.01);冠脉灌流液中SOD活性明显升高(P<0.01);MDA含量显著下降(P<0.01);药物组的PKC含量水平较模型组增多(P<0.05),药物组NCX mRNA的表达水平较模型组降低,存在显著差异(P<0.05)。结论钙拮抗剂Nifedipine对大鼠心肌缺血再灌注损伤有保护作用。Objective Study the protective effect of Calcium Antagonist Nifedipine,on myocardial ischemia-reperfusion injury in isolated rat hearts.Methods Rat myocardial ischemia-reperfusion injury model was established by consisting of a 30 min（reducing perfusion flow level of left and right coronary to 5% of the original irrigation flow） ischemic period and 2h reperfusion,the isolated rat hearts were perfused on modified Langendorff apparatus.The rats were divided into control group,ischemia/reperfusion（I/R） group,Nifedipine group,then detected the myocardial infarct area,the levels of SOD and MDA,and PKCδ were detected by immunoHistochemistry,Semi-quantitative RT-PCR was employed to measure the mRNA levels of NCX and SERCA2a.Results（1） Compared with the I/R group,the myocardial infarct area was significantly lower,（2） SOD activity increased significantly and MDA decreased significantly;（3） Nifedipine group,compared with model group,levels of PKC increased（P〈0.05）;（4）expression of NCX mRNA in Nifedipine group was lower than the model group,there were significant differences（P〈0.05）;expression of SERCA2α mRNA in Nifedipine group was significantly increased compared with the model group（P〈0.05）.Conclusion The calcium antagonist Nifedipine on myocardial ischemia-reperfusion injury has a protective effect.

关 键 词：硝苯吡啶(Nifedipine) 缺血/再灌注 心肌梗死 钠钙交换体(NCX) 

分 类 号：R965[医药卫生—药理学]