胶东地区耐甲氧西林金黄色葡萄球菌耐药性的研究  

Study on multi-drug resistance of clinical methicillin-resistant staphylococcus aureusin isolates in Jiaodong distrcit

作　　者：李笃军[1] 苏维奇[2] 朱元祺[3] 

机构地区：[1]烟台经济技术开发区医院检验科,山东烟台264006 [2]青岛市市立医院 [3]青岛大学医学院附属医院

出　　处：《中国实验诊断学》2012年第2期290-292,共3页Chinese Journal of Laboratory Diagnosis

基　　金：山东省科技发展计划项目(2009GG10002057)

摘　　要：目的了解胶东地区耐甲氧西林金黄色葡萄球菌(MRSA)的耐药情况。方法采用VITEK2compact微生物分析仪进行细菌鉴定和药物敏感试验;头孢西丁Kirby-Bauer法和青霉素结合蛋白2a胶乳凝集(PBP2a)试验对分离出的金黄色葡萄球菌进行MRSA确认;酸测定法和D试验测定MRSA产β-内酰胺酶和红霉素诱导克林霉素耐药实验。结果头孢西丁纸片扩散法与PBP2a胶乳凝集法对MRSA检出率无统计学上差别(P>O.05);331株MR-SA产β内酰胺酶率和克林霉素诱导试验阳性率分别为91.14%和16.62%;全部菌株对万古霉素、替加环素和利奈唑胺敏感,但对红霉素、克林霉素耐药率高达82.19%和77.34%。结论 MRSA耐药机制复杂,呈现多重耐药,应根据药敏结果选用抗生素。Objective To study the situation of methicillin-resistant staphylococcus aureus （MRSA） resistant to multi-antibiotics in the local area,Methods The identification and drug susceptibility test were performed by VITEK2 compact automatic microbial analysis system. The MRSA were verified by cafoxitin disk diffusion and penicillin binding protein 2a latex agglutination. The erythromycin inducible clindamycin resistanc was tested by D-test. Results There are no significance（P〉0.05） between cafoxitin disk diffusion and penicillin binding protein 2a latex agglutination for verified MRSA. 13-1actamase producing rate and the ratio of erythromycin inducible clindamycin resistance were 91. 14 %, 16.62 %, respectively. All S. aureus isolates were susceptible to vancomycin, tigecycline and linezolid, but the majority were resistant to erythromycin（82.19%） clindamycin （77.34%）. Conclusion MRSA isolates were resistant to multiple antibiotics. And there is an urgent needs on antibiotics be adopted according to antimicrobial susceptibility tests.

关 键 词：甲氧西林 金黄色葡萄球茵 头孢西丁 Β-内酰胺酶 

分 类 号：R446.5[医药卫生—诊断学]