海因酶产生菌SHNU01的包埋固定化研究  

Study on the immobilization of D-hydantoinase-producing strains by entrapment

作　　者：陈建波[1] 孙婧[1] 

机构地区：[1]上海师范大学,生命与环境科学学院,上海200234

出　　处：《生物学杂志》2012年第1期33-36,共4页Journal of Biology

基　　金：国家自然科学基金子课题(编号20773038、AL200723);上海师范大学科研项目(编号DKL713)

摘　　要：对从土壤中筛选得到的高选择性产D-海因酶菌株SHNU01的性质进行了研究并探讨了用PVA-硼酸法进行包埋固定的效果。研究结果显示:固定化后的细胞与游离细胞相比,最适反应温度由游离细胞的37℃上升为47℃,在此温度下固定化细胞的酶活可达到游离细胞的3倍;连续反应7批后,固定化细胞活力为初始活力的80%。固定化细胞在操作稳定性、贮存稳定性等方面较游离细胞也有较大提高。D-hydantoinase-producing strain SHNU01with high selectivity was screened by using double-agar-layer and millipore rapid screening methods from soil. The properties of this strain and the immobilization by entrapment in polyvinyl alcohol （PVA）-boric acid were explored. Results showed that the immobilized cells exhibited excellent ability of tolerating higher temperature 47℃ compared with free cells 37℃. Under such conditions, the activity of the immobilized cells could reach to about 3 times of that of the free cells. After 7 batches, the enzyme activity decreased to 80% of the initial.

关 键 词：D-海因酶 聚乙烯醇 固定化 

分 类 号：Q93-3[生物学—微生物学]