肺炎链球菌感染的猕猴肺及气管内IFN-γ蛋白与mRNA表达  

Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae

作　　者：罗启慧[1,2] 章瑶[1] 陈恋[1] 陈正礼[1,2] 曾文[1,2] 程安春[1,2,3] 

机构地区：[1]四川农业大学动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]四川农业大学实验动物工程技术中心/国家实验用猕猴种源基地,四川雅安625014 [3]四川农业大学预防兽医研究所,四川成都611130

出　　处：《浙江大学学报（农业与生命科学版）》2012年第1期48-54,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：教育部"长江学者和创新团队发展计划"创新团队资助项目(IRT0848);四川省国际科技合作与交流研究计划资助项目(2010HH0013);四川省科技厅应用基础研究资助项目(2011JY0054);国家科技重大专项资助项目(2011ZX09307-301)

摘　　要：采用常规苏木精-伊红(hematoxylin-eosim,HE)染色、免疫组织化学和组织原位杂交技术,观察感染肺炎链球菌的猕猴肺及气管组织的病理学变化与γ干扰素(interferon-γ,IFN-γ)在其中的蛋白与mRNA表达.结果表明:在光镜下感染组的肺呈现大面积充血、出血,淋巴细胞浸润及肺泡腔内出现浆液性渗出物;IFN-γ蛋白表达面积均高于健康组(P<0.05);IFN-γ蛋白产物在肺血管内的光密度值极显著高于健康组(P<0.01),在气管和肺的其他部位光密度值显著高于健康组(P<0.05);IFN-γmRNA在肺气管上皮层、肌层内阳性产物的光密度值均极显著高于健康组(P<0.01),肺泡隔、血管内、气管固有层内阳性产物的光密度值均显著高于健康组(P<0.05).说明肺炎链球菌感染的猕猴肺出现了明显的红色肝变期病理变化,且肺及气管中IFN-γ蛋白及mRNA表达显著增加,表明IFN-γ在肺炎链球菌感染猕猴肺及气管的免疫调节中起到了重要作用.To investigate the expression and distribution of interferon-γ（IFN-γ） in lung and trachea of macaques infected with Streptococcus pneumoniae,the histopathological changes of macaques＇ lung and trachea were studied by using hematoxylin-eosin（HE） stain,where the expression of IFN-γ was researched by immunohistochemistry and in situ hybridization.The results showed that a large area of lung was hyperemic and hemorrhagic,and there was lymphocytic infiltration in lung and some serous exudate in alveolar septum.The area of IFN-γ protein expression in infected macaques was higher than healthy group（P 0.05）.The optical density of IFN-γ protein in pulmonary intravascular space was significantly higher than that of healthy group（P 0.01）,and the optical density in other tissues was higher than that of healthy group（P 0.05）.The optical densities of IFN-γ mRNA in lung,tracheal epithelium and muscular layer were significantly higher than those of healthy group（P 0.01）,while in alveolar septum,lumen of blood vessel and lamina propria of tracheal were higher than those of healthy group（P 0.05）.As a result,the lung presented significant red hepatization in infected macaques,and the expression of IFN-γ protein and mRNA in lung and trachea increased significantly,indicating that IFN-γ might play an important role in immunoloregulation of macaques＇ lung and trachea infected by S.pneumoniae.

关 键 词：肺炎链球菌 Γ干扰素 免疫组化 组织原位杂交 肺 气管 猕猴 

分 类 号：Q954.6[生物学—动物学]