体外角质形成细胞生长DNA合成动态观测—银屑病细胞动力学探讨  

The Observation of DNA in Cultured Normal and Psoriastic Keratinocytes.

作　　者：陈佳[1] 赵焕章 陈廷梅[2] 

机构地区：[1]西安医科大学第二附属医院皮肤科,710004 [2]西安医科大学第二附属医院检验科

出　　处：《中国皮肤性病学杂志》2000年第2期89-91,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的　观察银屑病角质形成细胞体外生长合成 ,探讨其病因。方法　应用体外角质形成细胞培养技术对 18例银屑病患者受累、未受累的表皮角质形成细胞进行了培养 ,并将其角朊细胞在孵育期中的3 H TDR掺入率及标记指数与正常对照做了比较。结果　银屑病病人受累、未受累表皮角质形成细胞3 H TDR掺入率在孵育期的第 3～ 4天升高 ,与正常人相比均有显著差异 (P <0 .0 5 )。银屑病病人的受累、未受累表皮角质形成细胞的标记指数在孵育期的第 3～ 8天升高 ,与正常人相比均有显著差异 (P <0 .0 5 )。结论　认为银屑病病人角质形成细胞在体外其DNA合成明显高于正常人 ,而且这一特征性的增殖仅在原代培养中持续一较短的时期 ,但引起这一改变的根本原因还有待于进一步的研究。Objective To study the pathogensis of psoriasis,and find the difference between the cultured normal and psoriatic kerationcytes(KCs).Methods Primary KCs cultures,from 18 involved(PP)、uninvolved(NP) of psoriasis grown on DMEM medium which 3 H thymidine incorporative rate was compared with that of the nomal control.Result The 3 H thymidine incorporation of psoriatic KCs was higher than that of normal KCs in 3～4 days.After 4 weeks,however,no significant difference was observed between the virture proliferation parameters of nomal and psoriatic KCs(P>0.05).The LI of psoriatic KCs merely increased compard with normal KCs at 3～8 days. Conclusion The result indicats that charactcristic of proliferiation of psoriatic KCs may only be maintained over as short period of primary culture.

关 键 词：银屑病 角质形成细胞 DNA合成 细胞动力学 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]