环磷酰胺对小鼠精子发生的影响  

THE EFFECTS OF CYCLOPHOSPLAMIDE ON SPERMATOGENESIS IN MICE

作　　者：陈安[1] 孙鸿喜[1] 熊艾君[1] 

机构地区：[1]湖南中医学院基础课部,湖南长沙410007

出　　处：《湖南中医学院学报》2000年第1期2-3,21,共3页Journal of Hunan College of Traditional Chinese Medicine

基　　金：湖南省科委资助!92-24004

摘　　要：观察雄性小鼠经腹腔注射环磷酰胺（ＣＹ）后，在不同时期其精子及睾丸组织学的变化。结果：第３４、３６ｄ，精子计数下降（Ｐ＜０．０１）、畸形率增高（Ｐ＜０．０１）；第２１、２８、３４、３６ｄ，生精细胞数均减少（Ｐ＜０．０５或 Ｐ＜０．０１）；睾丸超微结构（第 ３６ｄ）的变化主要表现为各级生精细胞核膜的溶解、核的破坏及精子细胞顶体囊泡的异常，但紧密连接不受影响。提示ＣＹ通过对精原细胞及细线前期初级精母细胞产生毒害作用而影响精子发生。Cyclophosplamide(CY)were injected intrapertontally to male mice. On the 21. 28. 34.36 d after administration,the changes of sperm and testis tissue in mice were observed. The results showed that on the 34.36 d the total sperm count decreased(P<0. 01),and the deformity rate of sperm increased (P<0. 01 ),on the 21. 28. 34. 36 d the number of spermatogenic cells decreased(P<0. 05 or P<0. 01),the ultrastructural changes (36 d ) were mainly break of nuclear membrane, injury of nuclear in the spermatogenic cells and abnormal acrosomic vesicle in spermatid,but the tight junction were normal. The results indicated that directly injuring the spermatogonia and spermatocyte at DNA-synthetic phase was the way by which CY disturbed the mice's spermatogenesis.

关 键 词：环磷酰胺 精子 生精上皮 电镜检查 

分 类 号：R979.5[医药卫生—药品]