高效液相色谱法同时测定双黄消炎片中黄芩苷和黄芩素含量  

Simultaneous Determination of Baicalin and Baicalein in Shanghuangxiaoyan Tablets by HPLC

作　　者：徐占方 陈立柱 姜雪敏 孙菲 

机构地区：[1]黑龙江省鸡西市药品检验所,黑龙江鸡西158100

出　　处：《中国药业》2012年第4期28-29,共2页China Pharmaceuticals

摘　　要：目的建立同时测定双黄消炎片中黄芩苷和黄芩素含量的反相高效液相色谱法。方法色谱柱采用Agilent Eclipse XDB-C18柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸溶液为流动相(梯度洗脱),检测波长为277 nm,流速1.0 mL/min,进样量10μL,柱温35℃。结果黄芩苷质量浓度在5.678～141.95μg/mL范围内与峰面积有良好线性关系,r=1.000 0(n=7);黄芩素质量浓度在2.122～53.05μg/mL范围内与峰面积有良好线性关系,r=1.000 0(n=7)。黄芩苷平均回收率为99.32%,RSD为0.63%(n=6);黄芩素平均回收率为99.27%,RSD为0.48%(n=6)。结论该法操作简便、快速、结果准确,可用于双黄消炎片的质量控制。Objective To establish RP-HPLC method for simultaneous determination of Baicalin and Baicalein In shuanghuangxiaoyan tablets. Methods Using a column packed with Agilent Eclipse XDB-C18（250 mm ×4.6 mm,5 μm）. The mobile phase was methanol- 0. 1%H3PO4 by gradient elution and the detection wavelength was 277 nm,the flow rate was 1.0 mL/min,the injection volume was 10 μL,the column temperature was 35 ℃. Results The linear response of baicalin range from 5. 678 - 141.95 μg/mL, r = 1. 000 0（ n = 7）. The linear response of baicalein range from 2. 122 -53.05 μg/mL, r = 1. 000 0（ n =7）. The average recovery of baicalin was 99.32% , RSD=0. 63%（n=6）.The average recovery of baiealein was 99.27%,RSD=O. 48%（n=6）.Conelusion The method was found to be convenient, quick and accurate.

关 键 词：含量测定 黄芩苷 黄芩素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]