小鼠急性肺血栓栓塞症模型的建立与比较  

Establishment and comparative studies of acute thromboembolic pulmonary embolism mice models

作　　者：郑睿[1,2] 刘杰[1] 万钧[1] 姚建民[3] 徐洪涛[4] 

机构地区：[1]首都医科大学生理学系,100069 [2]第三军医大学 [3]北京军区总医院心肺中心,100700 [4]海军总医院心内科,北京100037

出　　处：《重庆医学》2012年第5期428-431,F0002,共5页Chongqing medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2009CB522107);国家自然科学基金资助项目(81070042);北京市自然科学基金资助项目(7082012);重大国际合作研究基金资助项目(30810103904)

摘　　要：目的通过评估不同方法诱导的肺血栓栓塞症小鼠模型效果,筛选建立操作简便、使用经济、成功率高的小鼠急性肺血栓栓塞症模型方法。方法制作全血血栓及白色血栓自体栓子,穿刺并注入小鼠右侧颈静脉,造成急性肺血栓栓塞症模型,分别于不同时间点检测相应生理、生化指标。结果经Mallory磷钨酸苏木精及HE染色证明,两种栓子制备肺血栓栓塞症模型的造模成功率为100%,栓子多处于肺动脉段及亚段水平,栓塞切实可靠;白色血栓栓塞组栓子较全血血栓组稳定性明显增高,自溶时间明显延长。结论白色血栓诱导的肺栓塞小鼠的病理状态更加稳定,且该急性肺血栓栓塞模型可以较好地模拟病理情况下这一疾病的病理、生理过程,为今后进一步研究肺血栓栓塞症的病理机制与筛选药物提供了一定的实验基础。Objective By ways of evaluating mice pulmonary embolism models induced by different methods,to screen the method to establish the acute pulmonary embolism mice models that is easy to operate and affordable with the high success rate.Methods The pre-operated whole blood emboli or white emboli were separately infused into the right jugular vein to induce acute thromboembolic mice models,and then the physiological parameters were tested at different time points.Results The successful rate of pulmonary embolism model constructing by the two kinds of emboli was up to 100%.Most emboli laid in the level of segments or subsegments of pulmonary arteries,and most importantly,the obvious fibrinolysis of the thrombi in white-autologous thrombi pulmonary embolism models was largely improved than the whole blood group.Conclusion By comparing two groups of models induced by different ways,we find that the white-emboli induced acute pulmonary embolism mice maintains a much more stable pathological state,and our way to establish models of acute pulmonary thromboembolism simulates its real physiological process to a great extent.Our work has laid a solid foundation for further studies of pathogenesis of pulmonary embolism and drug screening.

关 键 词：肺栓塞 小鼠 自体血栓 白色血栓 

分 类 号：R563.5[医药卫生—呼吸系统]