CD4^+CD25^+、CD4^+CD25^(hig)调节性T细胞和Foxp3 mRNA在婴幼儿喘息中的表达及意义  

Expression of regulatory CD4^+CD25^+ Treg,CD4^+CD25^(hig) Treg cells and Foxp3 mRNA in wheezing infants and its clinical significance

作　　者：彭力[1] 钟礼立[1] 黄寒[1] 厉娟[1] 梁沫[1] 李云[1] 

机构地区：[1]湖南省人民医院儿科,长沙410005

出　　处：《重庆医学》2012年第5期445-448,共4页Chongqing medicine

摘　　要：目的探讨婴幼儿喘息CD4+CD25+、CD4+CD25hig调节性T细胞(Treg)和叉头/翼状螺旋转录因子(Foxp3)mRNA的表达及意义。方法采用流式细胞术检测51例首次喘息婴幼儿外周血CD4+CD25+Treg和CD4+CD25hig Treg的比例,RT-PCR检测Foxp3mRNA的表达量,酶联免疫吸附法(ELASA)检测总IgE水平,并与正常婴幼儿对照。结果喘息婴幼儿外周血CD4+CD25+Treg、CD4+CD25higTreg占CD4+T细胞的百分比分别为(6.31+2.96)%和(3.52+1.46)%,均明显低于健康对照组(P<0.01);特应征喘息组CD4+CD25+Treg、CD4+CD25higTreg及Foxp3mRNA表达均低于非特应征喘息组(P<0.05)。喘息患儿CD4+CD25higTreg百分率及Foxp3mRNA表达与血清总IgE之间呈显著负相关(r=-0.75,r=-0.61,P<0.01),而CD4+CD25+Treg百分比与血清总IgE存在正相关(r=0.36,P<0.05)。结论 CD4+CD25+Treg、CD4+CD25hig Treg及Foxp3mRNA在婴幼儿喘息的发病中起重要作用。Objective To evaluate the changes of CD4＋CD25＋Treg,CD4＋CD25hig Treg and Foxp3 mRNA in peripheral blood from wheezing infantsMethods Fifty-one wheezing infants and twenty healthy volunteers were included in this study.The proportion of CD4＋CD25＋Treg and CD4＋CD25hig Treg population in total T cells was evaluated by flow cytometric analysis.The expression of Foxp3 mRNA was tested by flow cytometry.Total serum IgE of wheezy infants was detected by enzyme immunoassay.Results Compared with those of healthy control,the frequency of CD4＋CD25＋Treg and CD4＋CD25hig Treg in the peripheral blood from wheezing infants showed a significant increase（6.31＋2.96） % /（3.52＋1.46） %,P0.01,P0.01,respectively）.The expression of CD4＋CD25＋Treg,CD4＋CD25hig Treg and Foxp3 mRNA in peripheral blood from wheezing infants with atopy burden was lower than those from non-wheezing infants（P 0.05）.The correlation analysis showed that CD4＋CD25hig Treg（r=-0.75,P0.01） and Foxp3 mRNA（r=-0.61,P0.01） had significantly positive relation with total serum IgE,while CD4＋CD25＋Treg had significantly negative relation with total serum IgE（r=0.36,P0.05）.Conclusion CD4＋CD25＋Treg,CD4＋CD25hig Treg and Foxp3 mRNA play an important role in activation of wheezing infants.

关 键 词：T淋巴细胞 调节性婴幼儿喘息 调节性T细胞 叉头转录因子类 特应征 

分 类 号：R725.6[医药卫生—儿科]