FFAs对人网膜脂肪细胞脂联素分泌的影响及罗格列酮的干预作用  

Effect of FFAs on Adiponectin Secretion in Human Omental Adipocytes and Intervention by Rosiglitazone

作　　者：黄德斌 钟惠菊[2] 

机构地区：[1]长沙市第三医院内分泌代谢病科,湖南长沙410015 [2]中南大学湘雅医院内分泌代谢病科,湖南长沙410011

出　　处：《医学临床研究》2012年第1期47-49,共3页Journal of Clinical Research

摘　　要：[目的]观察游离脂肪酸（FFAs）对人成熟网膜脂肪细胞脂联素分泌的影响及罗格列酮（RSG）干预的效果.[方法]取6名外科手术女性患者的网膜脂肪组织,胶原酶消化分离纯化成熟脂肪细胞.细胞在FFAs（油酸∶软脂酸,2∶1）和（或）加用RSG的培基中培养不同的时间,酶联免疫吸附法测定培基中脂联素浓度.[结果]FFAs呈剂量及时间依赖性抑制网膜脂肪细胞脂联素分泌,各个干预组与对照组相比,脂联素降低有统计学意义;RSG可逆转此种抑制作用,高浓度（10 μmol/L）时逆转作用更强.[结论]FFAs能抑制体外培养的人网膜脂肪细胞脂联素分泌,但此种抑制作用可被RSG逆转.[Objective] To observe the effect of free fat acids（FFAs） on adiponectin secretion in human mature omental adipocytes and intervention by rosiglitazone（RSG）. [Methods] Omental adipose tissue was ob- tained from 6 female patients undergoing elective surgery, and then digested, isolated and purified into mature adipocytes by collagenase treatment. Adipocytes were cultured in FEAs（oleate： palmitate, 2： 1） and/or RSG medium for different time. The adiponectin level in the medium was measured by ELISA. [Results] FFAs in- hibited adiponectin secretion in omental adipocytes in a dose- and time-dependent manner. Compared with con- trol group, the decrease of adiponectin in intervention groups had statistical significance. RSG could reverse the inhibitory effect. Moreover, high dose of RSG （10umol/L） had more prominent reverse effect. [Conclusion] FFAs can inhibit adiponectin secretion in cultured omental adipocytes. The inhibitory effect of FFAs can be reversed by RSG.

关 键 词：脂细胞 降血糖药 

分 类 号：R977.15[医药卫生—药品]