包被抗人CD3单克隆抗体对CIK细胞生长影响的研究  

Study on influence of monoclonal antibody coated with anti-human CD3 on CIK cells growth

作　　者：王岩 刘蓉芳 晁玉琴 

机构地区：[1]河南省驻马店肿瘤医院医学生物细胞室,463000 [2]河南省驻马店肿瘤医院检验科,463000

出　　处：《检验医学与临床》2012年第4期413-414,417,共3页Laboratory Medicine and Clinic

摘　　要：目的通过对比包被和未包被抗人CD3单克隆抗体培养瓶中的杀伤细胞(CIK细胞)生长状态及培养一段时间后收集的细胞数,研究在临床常用培养CIK细胞的方法上,后续增加对培养瓶进行包被抗人CD3单克隆抗体的预处理后,对CIK细胞生长的影响。方法提取健康者的外周血单个核细胞(PBMC),无血清培养液调整好细胞浓度,在75cm2密封盖培养瓶中等量加入包被和未包被抗人CD3抗体,以及IFN-γ。培养24h后加入CIK混合刺激因子,以后每天观察细胞的生长情况,根据细胞的生长情况进行传代扩增(扩增至未包被的培养瓶中),第12天收集细胞进行比较。结果包被抗人CD3抗体的75cm2密封盖培养瓶培养的CIK细胞,在培养第3天即可进行传代扩增,以后每天都可进行扩增;用未包被抗人CD3抗体75cm2密封盖培养瓶培养的CIK细胞在第5天才可进行传代扩增,以后每隔1d可以进行扩增,第12天收集的细胞总数未包被的为1.2×109,包被的为3.3×109。结论包被细胞因子抗人CD3单克隆抗体对正常条件培养的CIK细胞生长状态和较短时间内收获的CIK细胞总量有很好的正影响。Objective To study the effects of monoclonal antibody coated with anti-human CD3 on quality and quantity of CIK cells cultured in culture flask by comparing the pretreatments with not coated anti-human CD3,based on common clinical cultural methods of CIK cell.Methods To extract peripheral blood mononuclear cell（PBMC） from healthy people,and adjust the certain concentrations of serum-free cell culture,add the same amounts of antibody coated with anti-human CD3 and not coated antibody and subsequently add IFN-γ in 75cm sealed cultural flask.After 24 h treatment,CIK mixed stimulating factor was added,the growth status was observed and recorded everyday.The cells were collected for comparison on 12 d.Results CIK cells with antibody coated by anti-human CD3 began to amplify after 3 d and every day of the amplification latter;CIK cells not coated with anti-human CD3 antibody began to amplify only after 5 d.The total number of collected cells was 1.2×109,for not coated treatment,and 3.3×109 for coating treatment.Conclusion The cytokine-coated anti-human CD3 monoclonal antibody in normal condition has a good positive influence on the growth of cultured CIK cells within the relatively short time.

关 键 词：杀伤细胞 包被细胞因子 细胞生长状态 

分 类 号：R979.5[医药卫生—药品]