Caspase-3在微囊藻毒素-LR诱导大鼠睾丸支持细胞凋亡中的作用  

Effect of Caspase-3 on Apoptosis of Sertoli Cells Induced by Microcystin-LR among Rats

作　　者：李超锋[1] 张丰泉[1,2] 辛红霞[3] 易丹[1] 赵仲麟[4] 袁超[4] 崔留欣[1] 张慧珍[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生与环境卫生学系,河南郑州450001 [2]嵊州市疾病预防控制中心卫生科 [3]河南省人民医院眼科研究所 [4]河南农业大学理学院

出　　处：《环境与健康杂志》2012年第2期164-165,F0003,共3页Journal of Environment and Health

基　　金：河南省基础与前沿技术项目(112300410070);河南省高等学校青年骨干教师资助计划(2011GGJS-012)

摘　　要：目的研究微囊藻毒素-LR(MC-LR)染毒对大鼠睾丸支持细胞凋亡形态以及半胱氨酸天冬氨酸蛋白酶-3(caspase-3)活力的改变。方法自健康18～20日龄SPF级SD雄性大鼠体内分离、培养睾丸支持细胞。用含MC-LR终浓度分别为0(对照)、1、10μg/ml的培养液培养48 h。采用Hoechst荧光染色法检测睾丸支持细胞的凋亡情况;采用caspase-3底物切除法检测caspase-3的活力。结果随着MC-LR染毒剂量的增高,大鼠睾丸支持细胞凋亡率升高。与对照组比较,仅10μg/ml MC-LR染毒的睾丸支持细胞caspase-3活力升高,差异有统计学意义(P<0.05);而1μg/ml MC-LR染毒睾丸支持细胞caspase-3活力略有降低,但差异无统计学意义。结论 MC-LR能诱导睾丸支持细胞发生凋亡,caspase-3在MC-LR诱导睾丸支持细胞凋亡中起重要调控作用。Objective To study the changes of apoptosis morphology and the effect of caspase-3 on the apoptosis induced by microcystin in rat Sertoli cells. Methods Sertoli cells were isolated and purified from youthful SD rats and the cell model was established. After exposure to MC-LR （0, 1, 10 μg/ml）,apoptbsis of Sertoli cells induced by MC-LR was detected using Hoechst fluorescent staining. The caspaure activity in Sertoli ceils was measured by cleavage of the caspase substrate. Results The number of apoptosis Sertoli cells progressively increased with the increasing MC-LR dosage. After Sertoli cells were exposed to 10 μg/ml MC-LR MG-LB,caspase-3 activity significantly mcreased. Conclusion MC-LR may reduce apoptosls of Sertoli cells. Caapase-3 may play an important role in the apoptosis of Sertoli cells induced by MC-LR.

关 键 词：微囊藻毒素-LR 睾丸支持细胞 细胞凋亡 半胱氨酸天冬氨酸蛋白酶-3 Hoechst荧光染色 

分 类 号：R994.6[医药卫生—毒理学]