N-乙酰基转移酶-2基因多态性和环境因素与女性乳腺癌的关系  

Association between Genetic Polymorphisms of N-acetyltransferase 2,Environmental Factors and Female Breast Cancer

作　　者：陈海燕[1] 李君[1,2] 蒋守芳[1,2] 刘坤[1] 赵刚[3] 牛凤玲[3] 张国志[4] 

机构地区：[1]河北联合大学公共卫生学院,河北唐山063000 [2]河北省煤矿卫生与安全实验室 [3]唐山市人民医院 [4]河北联合大学附属医院

出　　处：《环境与健康杂志》2012年第2期173-176,共4页Journal of Environment and Health

摘　　要：目的分析N-乙酰基转移酶-2(NAT2)基因多态性和环境因素与女性乳腺癌的关系。方法采用以医院为基础的1∶1配对的病例-对照研究,收集唐山市原发性乳腺癌新发女性患者48例与相应非肿瘤患者48例。以问卷调查收集各研究对象的饮食习惯和生活方式、生理生育、环境暴露和职业接触、既往病史、心理等信息。采用聚合酶链反应-限制性片段多态性(PCR-RFLP)技术检测NAT2的野生型等位基因WT、突变型等位基因M1、M2和M3突变频率,分析NAT2基因多态性和环境危险因素与女性乳腺癌的关系。结果被动吸烟大于或等于10年(OR=3.957,95%CI:1.589～10.002)、居住地环境污染(OR=33.571,95%CI:4.270～263.967)、职业接触(OR=9.400,95%CI:1.127～78.405)、烹调时使用排油设备(OR=0.177,95%CI:0.060～0.529)、农药的使用(OR=28.200,95%CI:3.576～222.389)等是乳腺癌的环境危险因素。携带M2、M3等位基因可能是乳腺癌的危险因素,OR值分别为2.563(95%CI:1.155～5.707)和2.083(95%CI:1.068～4.062),而M1等位基因频率在病例组与对照组的分布差异无统计学意义(χ2=0.447,P>0.05)。病例组与对照组突变杂合子基因型(WT/Mx)分布频率差异无统计学意义(χ2=0.021,P>0.05),而两组间突变纯合子(Mx/Mx)分布频率差异有统计学意义(OR=3.545,95%CI:1.141～11.015)。慢乙酰化表型者患乳腺癌的风险是快乙酰化表型者的3.364倍(χ2=7.599,P<0.05)。分层分析发现NAT2慢乙酰化表型与被动吸烟大于或等于10年存在交互作用(OR=9.917,95%CI:1.597～61.597)。结论 NAT2基因多态性和部分环境危险因素与乳腺癌发病存在统计学关联。Objective To explore the association between N-acetyhransferase 2 （NAT＇2） genetic polymorphisms, environmental factors and female breast cancer in Tangshan area, Hebei, China. Methods A 1：1 matched case-control study was conducted in which 48 primary breast cancer cases and 48 hospital controls were selected. A questionnaire survey was administered to collect basic information. MI, M2, M3 alleles of NAT2 were detected by PCR-RFLP technique. The relationship among NA T2 genetic polymorphisms, environmental factors and breast cancer was analyzed. Results As the results of conditional logistic regression showed, passive smoking exceeded 10 years （OR =3.957,95% CI：1.589-10.002）, residence environmental pollution（OR=33.571,95%Cl：4.270-263.967）,occupational exposure （0R=9.400,95%CI：1.127-78.405）, the using of taking lampblack equipment when cooking （OR =0.177, 95%CI： 0.060-0.529） and pesticide （OR =28.200,95%CI：3.576- 222.389）, and etc, were all risk factors for breast cancer. Can＇ying with alleles M2 （0R=2.563,95%CI： 1.155-5.707） or M3 （OR= 2.083,95%CI： 1.068-4.062） might be risk factors of breast cancer, but no. difference of MI frequencies between the case and control group were found （X2=0.447, P〉0.05）. It showed no difference of the frequencies of genotype WT/Mx between case and control group （X2=0.021,P〉0.05）, while the difference of genotype Mx/Mx in the two groups was significant （OR=3.545, 95%CI： 1.141-11.015）.h showed that the risk of breast cancer with slow acetylation type was 3.364 times higher than rapid type （95%CI 1.396-8.104）. By stratified analysis, the interaction was observed between NAT2 genetic polymorphisms and passive smoking exceeded 10 years （OR=9.917, 95%C1：1.597-61.597）. Conclusion NA T2 genetic polymorphisms and environmental factors may be associated with the risk of breast cancer.

关 键 词：乳腺癌 NAT2基因多态性 环境因素 交互作用 

分 类 号：R181.3[医药卫生—流行病学]