星点设计-效应面法优选苦参提取工艺  

Optimization of Extraction Technology for Sophora flavescens by Central Composite Design-Response Surface Methodology

作　　者：涂瑶生[1,2] 李绍林[1,2] 孙冬梅[1,2] 张建军[2] 江志强[1,2] 付建武[1,2] 

机构地区：[1]广州中医药大学,广州510405 [2]广东省中医研究所,广州510095

出　　处：《中国实验方剂学杂志》2012年第5期34-37,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优化苦参的提取工艺。方法:采用单因素和星点设计-效应面法,以苦参碱、氧化苦参碱、苦参总黄酮提取率和干膏得率为考察指标对苦参提取工艺进行优化。结果:优选的最佳提取工艺为加11倍量65%乙醇提取2次,每次65min。结论:优选的提取工艺合理、稳定、可行。Objective：To optimize extracting technology of Sophora flavescens.Method： Taking extraction rate of matrine,oxymatrine,total flavonoids from S.flavescens radix and yield of dry extract as index,extraction process of S.flavescens was optimized by single factor test and central composite design-response surface methodology.Result： Optimum condition of extraction technology was extracted 2 times with 11-fold,the amount of 65%ethanol for 65 minutes each time.Conclusion： Optimum extraction process was reasonable,stable and feasible.

关 键 词：苦参碱 氧化苦参碱 总黄酮 星点设计-效应面法 提取工艺 

分 类 号：R283.6[医药卫生—中药学]