忍冬苷的合成及其对雷公藤甲素诱导肝损伤的保护作用  

Synthesis of Lonicerin and its Protective Effect on Liver Cell Injured by Triptolide in vitro

作　　者：刘芳[1] 舒积成[2] 潘景行[2] 张锐[2] 刘建群[2] 

机构地区：[1]江西萍乡高等专科学校化工系,江西萍乡337055 [2]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2012年第5期72-75,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960495)

摘　　要：目的:研究忍冬苷的合成方法及其对雷公藤甲素诱导的肝损伤的保护作用。方法:以来源丰富的新橙皮苷为原料,经I2脱氢,AlCl3脱甲基,制得忍冬苷;采用雷公藤甲素诱导的人系L02肝细胞损伤模型评价忍冬苷的保肝作用。结果:合成了忍冬苷并发现其对雷公藤甲素诱导的肝损伤具有很好的保护作用。结论:合成方法简便可行、收率高、成本低,适合工业化生产;忍冬苷对雷公藤甲素诱导的肝损伤具有很好的保护作用。Objective：Study on the synthesis of lonicerin and its protective effect on liver cell injured by triptolide.Method： Lonicerin was synthesized with neohesperidin through dehydrogenation and demethylation by iodine and aluminum trichloride respectively and its protective effect on L02 cell injured by triptolide in vitro was evaluated by 3-（4,5）-dimethylthiahia20（-z-y1）-3,5-di-phenyterazoliumromide（MTT） assay for the first time.Result： Lonicerin was synthesized and had the protective effect on L02 cell injured by triptolide in vitro.Conclusion： The synthetic route for lonicerin with high yield was simple,feasible,economic and convenient for industrial manufacture.Lonicerin had the protective effect on L02 cell injured by triptolide.

关 键 词：忍冬苷 合成 雷公藤甲素 肝损伤保护 

分 类 号：R284.3[医药卫生—中药学] R285.5[医药卫生—中医学]