乌贼墨肽聚糖的制备工艺与体外抗前列腺癌研究  

The Peptidoglycan Extracted from Ink of Sepia and the Inhibition against the Growth of Prostatic Cancer in Vitro

作　　者：郑玉寅[1] 杨永芳[1] 丁国芳[1] 黄芳芳[1] 杨最素[1] 郁迪[1] 

机构地区：[1]浙江海洋学院食品与药学学院,浙江舟山316004

出　　处：《时珍国医国药》2012年第1期111-113,共3页Lishizhen Medicine and Materia Medica Research

基　　金：浙江省科技厅重大项目(No.2010C13009);浙江省自然科学基金(No.Y3100129;No.Y2100805);浙江省教育厅研究生创新科研项目(No.YK2008072);浙江省新苗人才计划项目(No.2010R411039);浙江省舟山市科技计划项目(No.092016)

摘　　要：目的研究乌贼墨肽聚糖的提取和分离纯化工艺,探讨其基本的结构组成,并研究它的抗前列腺癌活性。方法用Tris-HCl缓冲液提取、丙酮脱脂和Sevag试剂除蛋白方法提取出粗肽聚糖。将提取物用超滤、DEAE-52离子交换柱、G-75凝胶柱进行分离纯化,高效液相色谱和SDS-PAGE凝胶电泳进行纯度检测,经红外扫描,确定该组分具有肽聚糖的一般吸收特征,以及检测它的氨基酸组成。最后采用MTT法检测纯化后得到的肽聚糖对DU-145和PC-3细胞的增殖的影响。结果最终水提得到的肽聚糖的纯化物对DU-145和PC-3细胞均有较明显的增殖抑制效应,且抑制作用具有明显量效依赖关系。结论该法提取的乌贼墨肽聚糖具有明显的抗前列腺癌作用。Objective To extract the anti-prostatic cancer（Pca） effect of peptidoglycan from ink of Sepia.. Methods The peptidoglycan was extracted by Tris-HCl buffer solution,acetone degreasing and sevag reagent except protein.Peptidoglycan was purified by ultrafiltration,DEAE-52 and Sephadex G-75 column chromatography and the purity of peptidoglycan was demonstrated by HPLC and SDS-PAGE electrophoresis,and structure characterization by IR.The anti-Pca peptidoglycan were evaluated by MTT. Results The results of MTT showed that peptidoglycan from ink of Sepia significantly inhibited the proliferation of DU-145 and PC-3 cells. Conclusion The peptidogly can from the ink of Sepia shows obvious anti-Pca activity.

关 键 词：乌贼墨 肽聚糖 前列腺癌 抗肿瘤 

分 类 号：R979.1[医药卫生—药品] TS225[医药卫生—药学]