广西甜茶制剂中甜茶素含量的RSLC-DAD测定  

Quantification of Rubusoside in Preparations of Rubus Suavissimus by RSLC-DAD

作　　者：樊兰兰[1] 韦玮[1] 何丽丽[1] 曹芬[1] 缪剑华[1] 

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023

出　　处：《时珍国医国药》2012年第1期129-131,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区科技攻关项目(No.0718006-3c);广西壮族自治区科技成果推广与产业化示范项目(No.0632005-1)

摘　　要：目的建立RSLC-DAD测定广西甜茶制剂中甜茶素含量的方法。方法色谱柱为Dionex RSLC 120 C18柱(50 mm×2.1 mm,2.2μm),流动相为乙腈-水(36∶64),检测波长205 nm,柱温40℃,流速0.3 ml/min,进样量2μl。结果甜茶素的线性范围为0.03～0.6μg;加样回收率为99.1%,RSD为0.97%(n=9);甜茶素在21份中国和日本市售甜茶制剂中的含量范围为0～63.79 mg/g。结论该检测方法简便、快速、可靠,可作为测定甜茶制剂中甜茶素含量的方法。Objective To improve the quality standard of Jiannao Bushen pills for better control of the quality of drugs. Methods The TLC was employed on Radix Angelicae Sinensis,Fructus Forsythiae,Cinnamon,Ramulus Cinnamomi,Largehead Atractylodes Rh and Radix Ginsengrubra for identification;the content of paeoniflorin in white paeony root was determined by HPLC.A Dikma Diamonsil C18 column（250 mm×4.6 mm,5μm）was adopted with acetonitrile-0.4% phosphoric acid solution（15：85） at the flow rate of 1.0 ml·min-1,and the detection wavelength was 230nm. Results TLC spots were clear and the black test showed no interference.Paeoniflorin in the range of 0.18648～0.93240 μg showed good linearity（r=1）,the average recovery was 96.46% and RSD was 1.42%（n=6）. Conclusion This method is accurate and reliable,and can be used for the preparation of quality control.

关 键 词：广西甜茶 甜茶素 含量测定 RSLC-DAD 

分 类 号：R284.2[医药卫生—中药学]