紫外分光光度法测定天麻中的微量元素硒  

Study on the Ultraviolet Spectrophotometric Determination of Selenium in Gastrodia

作　　者：黄锋[1] 张永瑞[1] 

机构地区：[1]云南省昭通市疾病预防控制中心,云南昭通657000

出　　处：《云南化工》2011年第6期36-38,共3页Yunnan Chemical Technology

摘　　要：目的:建立紫外分光光度法测定天麻中微量元素硒的方法。方法:采用微波消解技术处理样品,加入HCl将Se6+还原成Se4+,Se4+与邻苯二胺络合,用甲苯萃取,紫外分光光度法测定天麻中的微量元素硒。结果:相关系数r=0.9994,加标回收率为96.6%～103.7%,最大吸收波长为348 nm,硒含量在0.01μg/kg～8.00μg/kg范围内符合比尔定律。结论:该方法简单快速、灵敏度高、准确度好和检出费用低等优点,适合基层实验室对食品中微量元素硒的测定。Objective： to establish a method for determination of selenium content in gastrodia by ultraviolet spectrophotometry.Methods： the use of microwave digestion technique for processing samples,addition of HCl,in which the Se6＋ was reducted to Se4＋ and tetravalent selenium complexed with o-Phenylenediamine,extraction with toluene,using ultraviolet spectrophotometric method to determine selenium in gastrodia.Results： the correlation coefficient r=0.9994,the recovery of standard addition is 96.6%～103.7%,wavelength Max.absorbance is 348 nm,selenium content in the 0.01 μg/kg～8.0 μg/kg range meets Beer law.Conclusion： the experimental results show the method is simple,rapid,high sensitivity,the result is accurate,the test cost is low,and it is easy for the determination of selenium in the basic unit laboratory.

关 键 词：紫外分光光度法 邻苯二胺 天麻 硒 微波消解 

分 类 号：R284[医药卫生—中药学]