运动性低血色素大鼠骨骼肌有氧氧化能量代谢系统的变化研究  

The Changes of Hepatic,Muscle Glycogen and Succinate Dehydrogenase 3mRNA Expression in Skeletal Muscle of Sport Low Hemoglobin Rats

作　　者：张毅[1] 

机构地区：[1]汕头大学体育部,广东汕头515063

出　　处：《北京体育大学学报》2011年第12期49-51,共3页Journal of Beijing Sport University

基　　金：科技部重点课题:运动性机能低下--运动性贫血的防治(子课题)(课题号:2002BA904804-07)

摘　　要：为进一步研究改善贫血状况和采用恢复手段促进骨骼肌的快速恢复打下基础。方法:将20只Wistar大鼠随机分为安静对照组(n=10)和运动组(n=10)。采用7周递增负荷跑台运动建立运动性低血色素大鼠模型,建模成功后第二天宰杀大鼠,取骨骼肌和肝脏测定肌糖原、肝糖原含量,并测定骨骼肌的SDH活性和UCP3mRNA表达。结果:1)7周递增负荷跑台运动导致了运动大鼠红细胞计数、血红蛋白、红细胞压积显著降低(P<0.05),建模成功;2)运动性低血色素并未影响大鼠肌糖原和肝糖原的恢复(P>0.05);3)运动性低血色素大鼠骨骼肌SDH活性非常显著降低(P<0.01),使得UCP3mRNA表达非常显著增多(P<0.01)。结论:运动性低血色素不影响肌糖元和肝糖原的恢复速度,但是可以显著降低骨骼肌琥珀酸脱氢酶的活性,提示运动性低血色素状态可能使骨骼肌有氧氧化能力降低;运动性低血色素大鼠骨骼肌UCP3mRNA表达显著增加,可能与UCP发挥运动抗氧化的快速应答有关。To observe the changes of hepatic,muscle glycogen and succinate dehydrogenase 3mRNA expression in skeletal muscle of sport low hemoglobin rats and to provide a reference for further study on the recovery of the skeletal muscle motor capacity with sports anemia.Method：20 Wister rats were divided into two groups： silent control group（n=10） and training group（n=10）.After seven weeks increase mental treadmill exercise,the model of sports anemia was built up.All rats were killed during 24H after training.The Glycogen in liver and skeletal muscle,SDH activity and expression of UCP3mRNA were to be detected and observed.Results：1）Compared with silent control group,the RBC,Hb and HCT of training group decreased significantly（P0.05）,so the model was successful;2）There was no significant change in the recovery of the muscle and hepatic glycogen in sports anemia rats（P0.05）;3）In sports anemia status,the SDH activity decreased highly significantly（P0.01）;4）Expression of UCP3mRNA were increased significantly in sports anemia rats（P0.01）.Conclusions： 7 weeks increasing treadmill exercise can make rats appear symptoms of low hemoglobin;Sport low hemoglobin did not affect recovery speed of muscle and hepatic glycogen;the activity of SDH significantly reduce,and increased expression of UCP3mRNA.This may be the reasons that sport low hemoglobin can decrease the rate of athletic aerobic oxidation.

关 键 词：运动性低血色素 骨骼肌 糖原 琥珀酸脱氢酶 骨骼肌解偶联蛋白3 

分 类 号：G804.7[文化科学—运动人体科学]