珠眉海棠(Malus zumi Mats)盐应答MzDREBb基因的克隆与功能研究  

Cloning and characterization of salt-responding MzDREBb gene from Malus zumi Mats

作　　者：黄黎芳[1] 李擎天[1] 蒋玉妆[1] 陈浩[1] 孙扬吾[1] 孔瑾[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《中国农业大学学报》2011年第6期76-82,共7页Journal of China Agricultural University

基　　金：国家自然科学基金项目(30600416;31171940);北京市自然科学基金项目(5072027;6112012);国家"973"计划项目(SQ2011CB016411)

摘　　要：通过microarray(微列阵芯片)分析,从珠眉海棠盐胁迫cDNA文库中分离得到盐诱导的MzDREBb基因全长cDNA序列,研究苹果属植物DREB转录因子在胁迫中的作用。结果表明:MzDREBb全长1 185bp,开放阅读框共714bp,5′-UTR和3′-UTR的长度分别是121和350bp;MzDREBb编码237个氨基酸,有一个AP2/ERF结构域;系统分类将MzDREBb归入DREB家族的A-5亚组;MzDREBb定位在细胞核中,具有转录激活活性;半定量RT-PCR表明MzDREBb基因受到盐诱导。超表达MzDREBb基因的拟南芥耐盐能力增强。以上结果表明MzDREBb基因在盐胁迫应答中起重要作用。The DREBs are transcription factors which play central roles in abiotic stress response.Malus zumi,an apple rootstock,can survive 0.6% NaCl.The full-length cDNA sequence of MzDREBb gene was isolated from a cDNA library of Malus zumi by microarray analysis.The full-length cDNA of MzDREBb was 1 190 bp.Its open reading frame of 714 bp encoded a protein of 237 amino acids.The 5＇-UTR and the 3＇-UTR were 121 and 355 bp respectively.Base on multiple sequence alignment and phylogenetic characterization,MzDREBb was classified into A-5 subgroup of the DREB subfamily.The MzDREBb was localized in the nucleus,which exhibited transactivation activity in yeast two-hybrid system.MzDREBb was induced by either stress of high salinity or low temperature,but not by abscisic acid（ABA）.Transgenic Arabidopsis over-expressing MzDREBb could enhance salt tolerance.In conclusion,MzDREBb is involved in salt response through an ABA-independent pathway in Malus zumi.

关 键 词：珠眉海棠 DREB转录因子 盐胁迫 功能研究 

分 类 号：S661.4[农业科学—果树学]