中药方剂白头翁汤抗细菌内毒素的作用研究  

Study on the antiendotoxin action of Pulsatillae Decoction

作　　者：胡屹屹[1] 穆祥[2] 胡元亮[3] 

机构地区：[1]江苏省农业科学院兽医研究所,南京210014 [2]北京农学院兽医学中医药北京市重点实验室,北京102206 [3]南京农业大学动物医学院,南京210095

出　　处：《中国农业大学学报》2011年第6期132-136,共5页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30972209)

摘　　要：探讨中药方剂白头翁汤对细菌内毒素(LPS)的治疗和预防作用,取中药饮片按常规方法煎制成生药质量浓度为1g/mL的白头翁汤药液。分高(1g/mL)、中(0.5g/mL)、低(0.1g/mL)3个剂量给小鼠灌胃,每天1次,连续5d。第1次给药前3h和最后1次给药后3h分别腹腔注射LPS(9mg/kg)。记录LPS攻毒后48h内小鼠的死亡率,同时测定各组小鼠的体重、血液指标及器官指数。结果显示,预防高剂量组的小鼠死亡率极显著低于LPS对照组,差异极显著,治疗高剂量组和预防中剂量组显著低于LPS对照组,差异显著。治疗高剂量组和预防高剂量组的小鼠体重在24h时显著高于LPS对照组,差异显著。治疗高剂量组的小鼠白细胞数目、血红蛋白浓度、平均血红蛋白和血小板升高数量较LPS对照组均差异显著。治疗高剂量组小鼠的肝脏、脾脏和肠管指数显著低于LPS对照组,均差异显著。表明中药方剂白头翁汤具有明显的抗大肠杆菌内毒素作用。The purpose of this research was to investigate the antiendotoxin action of Pulsatillae Decoction（PD）.PD was extracted and condensed into the decoction at the crude drug concentration of 1 g/mL.Mice in prevention groups were challenged with 9 mg/kg lipopolysaccharide（LPS） by peritoneal injection after the treated with PD at three different concentrations（0.1,0.5 and 1 g/mL） for 5 days,and differently,mice in treatment groups were challenged with 9 mg/kg LPS by peritoneal injection before treated with PD at three concentrations（0.1,0.5 and 1 g/mL） for 5 days.The determined items and results were as follow：compared with LPS control group,the mortality were significantly decreased in the middle-and high-dose prevention groups and high-dose treatment group;the body weight were significantly increased in the high-dose treatment and prevention groups at 24 h;the WBC,HGB,MCH and PLT were significantly increased in the high-dose treatment group;the index of liver,spleen and intestinal were significantly decreased in the high-dose treatment group.It suggested PD had significant antiendotoxin effect

关 键 词：白头翁汤 小鼠 内毒素 死亡率 血液指标 脏器指数 

分 类 号：S853.74[农业科学—临床兽医学]