Effects of combined administration of furosemide and kanamycin on rat auditory nerve  

作　　者：GUO Weiwei YUAN Fen-qian LIU Hui-zhan YANG Shi-ming 

机构地区：[1]Department of Otolaryngology, Head ＆ Neck Surgery, Institute of Otolaryngology, Chinese PLA General Hospital, Beijing 100853, P.R. China [2]Department of Head and Neck Surgery, Jiangxi Cancer Hospital, Nanchang 330029, China

出　　处：《Journal of Otology》2011年第1期26-30,共5页中华耳科学杂志（英文版）

基　　金：supported by grants from the Major State Basic Research Development Program of China(973 Program)(#2011CBA01000);National Hi-Tech Research and Development Program of China(863 Program)(#2007AA02Z150)to SMY;the National Natural Science Foundation of China(NSFC)(#30871398,30730040,30628030)to SMY;National Eleventh Scientific Program(2008BAI50B08,2007BAI18B12,2007BAI18B14)to SMY

摘　　要：Objective To determine the effects of combined administration of furosemide and kanamycin on inner ear structures and the auditory nerve in rats. Methods The rats in the treatment group received intravenous injections of combined furosemide and kanamycin sulfate, and the rats in the normal control group received no treatment. The auditory brainstem response （ABR） test was carried out 7 days after drug administration to determine the effects of drug administration on hearing. Cochlear slice and cochlear wholcmount were prepared after 7 days of drug treatment. Results After 7 days of drug administration, ABR thresholds were significantly higher in the treatment group than in the control group and neurofilaments were significantly reduced, although the number of spiral ganglia showed no decrease and there were no signs of supporting cell injury. Conclusions Combined administration of furosemide and kanamyein sulfate has an apparent synergistic ototoxic effect. Although spiral ganglion damage may not be apparent within a short time period of drug administration, damage to auditory nerve fibers is obvious.Objective To determine the effects of combined administration of furosemide and kanamycin on inner ear structures and the auditory nerve in rats. Methods The rats in the treatment group received intravenous injections of combined furosemide and kanamycin sulfate, and the rats in the normal control group received no treatment. The auditory brainstem response （ABR） test was carried out 7 days after drug administration to determine the effects of drug administration on hearing. Cochlear slice and cochlear wholcmount were prepared after 7 days of drug treatment. Results After 7 days of drug administration, ABR thresholds were significantly higher in the treatment group than in the control group and neurofilaments were significantly reduced, although the number of spiral ganglia showed no decrease and there were no signs of supporting cell injury. Conclusions Combined administration of furosemide and kanamyein sulfate has an apparent synergistic ototoxic effect. Although spiral ganglion damage may not be apparent within a short time period of drug administration, damage to auditory nerve fibers is obvious.

关 键 词：KANAMYCIN FUROSEMIDE deafness model hair cell injury 

分 类 号：S858.31[农业科学—临床兽医学] Q959.833[农业科学—兽医学]