水稻早抽穗突变体ehd(t)的遗传分析和分子定位  

Genetic Analysis and Molecular Mapping of A Early Heading Date Mutant(ehd(t))in Rice

作　　者：李万昌[1] 姜丽娜[1] 王俊伟[1] 余娇娇[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《华北农学报》2011年第6期55-58,共4页Acta Agriculturae Boreali-Sinica

基　　金：河南省科技攻关资助项目(102102310318);河南省教育厅资助项目(2010A210021)

摘　　要：水稻品种的抽穗期是重要农艺性状之一,鉴定和定位抽穗期基因,分析其遗传效应对改良水稻抽穗期至关重要。在水稻品种Nipponbare中发现一个早抽穗植株,经过多代自交获得稳定的早抽穗突变株,突变体ehd(t)比Nipponbare早抽穗15 d。遗传分析表明,该突变受1对显性核基因控制,暂命名为EHD(t)。通过2个F2定位群体,利用微卫星标记将EHD(t)定位于第8号染色体RM223和RM584之间,其遗传距离分别为5.2 cM和6.1 cM。Rice heading date is an important agronomic trait.Mapping heading date gene is important for rice improvement.A rice（Oryza sativa L.）mutant with a early heading date,designated as edh（t）,was found from a japonica rice variety Nipponbare.This early heading date mutant phenotype was stably expressed through successive five self-crossed generations,and its heading date was earlier 15 days than that in wild-type.Genetic analysis showed that the phenotype of edh（t）was controlled by a single dominant nuclear gene,temporarily designated as EHD（t）.By means of molecular marker technique,the EHD（t） gene was mapped to an interval between two SSR markers RM223 and RM584 on chromosome 8 with two F2 segregating population,with genetic distances of 5.2 cM and 6.1 cM to each marker respectively.This is the basis for marker-assisted selection and map-based coloning this EHD（t） gene.

关 键 词：水稻 早抽穗基因 遗传分析 基因定位 

分 类 号：Q78[生物学—分子生物学]