HMC毒素诱导玉米同核异质B37原生质体细胞凋亡的检测  

The Detection of Apoptosis in Protoplasts from B37 Homocaryotic Multicytoplasm Genotype Maize Induced by HMC-Toxin

作　　者：马春红[1] 李秀丽[1,2] 董文琦[3] 及增发[3] 李晓煜[1] 戴志刚 王立安[2] 贾银锁[1] 

机构地区：[1]河北省农林科学院遗传生理研究所,河北省植物转基因中心,河北石家庄050051 [2]河北师范大学生命科学学院,河北石家庄050016 [3]河北省农林科学院,河北石家庄050051 [4]湖北省土壤肥料工作站,湖北武汉430064

出　　处：《华北农学报》2011年第6期136-140,共5页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金项目(C2011301010);河北省人才工程培养经费(冀人社字[2010]353号);河北省科学技术研究与发展计划项目(10393912D);科技部国际科技合作项目(2006DFB02480);农业部“948”项目(2008-Z20;2011-G1)

摘　　要：以B37-C和B37-N的玉米原生质体和叶片为试材,研究HMC毒素对两种同核异质玉米B37细胞凋亡的诱导作用。经Hoechst33258荧光染色表明:HMC毒素处理后的玉米原生质体均出现细胞凋亡现象,在荧光显微镜下可观察到凋亡小体、染色质边集或环状染色质等凋亡特征。原生质体的细胞凋亡率,B37的C细胞质显著高于N细胞质玉米,且凋亡率随处理浓度和处理时间的增加而上升。用HMC毒素处理玉米叶片,明显出现DNA-ladder现象时所需的毒素浓度C、N两种细胞质均为200μg/mL;但C细胞质所需的时间为6 h,而N细胞质则需9 h。用荧光显微观察与凝胶电泳分析所获得的结果一致,均表现出C细胞质对毒素较为敏感,先出现细胞凋亡;N细胞质对毒素的敏感性低于C细胞质,出现细胞凋亡的时间较晚。Apoptosis in protoplasts and leaves from Homokaryon maize B37 treated by HMC toxin were studied.The apoptotic features such as apoptotic bodies,chromatin margination or ring chromatin were found after the treatment stained by Hoechst33258.When treated by the same concentration of HMC toxin,the apoptosis rate of B37-C maize protoplasts were significantly higher than B37-N.In addition,the apoptosis rate was increased when the treatment time and concentration increasing.For the leaves of maize B37-C,the obvious DNA ladder was appeared with treatment concentration：200 μg/mL;treatment time：6 h.For the leaves of maize B37-N,the obvious DNA ladder was appeared with treatment concentration：200 μg/mL;treatment time：9 h which was longer than leaves of maize B37-C.Apoptosis in protoplasts from Homokaryon maize B37 were same to the leaves.B37-C was more sensitive than B37-N.

关 键 词：玉米小斑病菌C小种毒素 细胞凋亡 Hoechst33258染色 DNA梯状条带 

分 类 号：S513.01[农业科学—作物学]