转基因棉对棉蚜生长发育及其主要代谢物质含量的影响  

Effects of Transgenic Insect-resistant Cotton on Development and Metabolite Content of Cotton Aphid Aphis gossypii

作　　者：薛丽[1] 肖达[1] 卢延[1] 宋敦伦[1] 高希武[1] 

机构地区：[1]中国农业大学农学与生物技术学院昆虫系,北京100193

出　　处：《华北农学报》2011年第4期115-120,共6页Acta Agriculturae Boreali-Sinica

基　　金：转基因生物新品种培育重大专项(2008ZX08012-04)

摘　　要：近年来,转基因棉田非靶标害虫种群数量呈明显的上升趋势,棉蚜是转Bt(Bacillus thuringiensis)基因棉田发生危害较严重的非靶标刺吸性害虫之一。在实验室内,棉蚜分别用转Bt基因棉和亲本常规棉饲养40代以上,采用酶联免疫法(ELISA)检测转Bt基因棉SGK321上棉蚜体内的Bt蛋白含量,研究转Bt基因棉对棉蚜生长发育和体内3种主要代谢物质脂类、蛋白质和糖含量的影响。研究结果表明,长期饲养于转Bt基因棉上的棉蚜体内能检测到痕量的Bt蛋白。与亲本常规棉相比,转Bt基因棉上棉蚜寿命延长,产蚜数减少,但差异不显著。生命表参数也无显著差异。转Bt基因棉上的棉蚜体内总蛋白含量、三油酸甘油酯含量增加,总糖含量减少,但与亲本常规棉相比差异不显著。初步结果表明从长期效应看,SGK321对棉蚜的生长发育、主要代谢物质含量均无显著影响。In recent years,the population densities of non-target pests in transgenic Bt cotton fields were obviously increased.Cotton aphid Aphis gossypii（Homoptera： Aphidae） was one of the seriously non-target pests in transgenic Bt cotton field.One A.gossypii strain was respectively reared on transgenic Bt cotton line SGK321,expressing Cry1Ac insecticidal protein,and its transgenic parent cotton cultivar Shiyuan321 for more than forty generations in the laboratory.The contents of Bt protein,carbohydrate,lipid,protein and life table parameters in A.gossypii,were measured and compared on two host plants SGK321 and Shiyuan321.The results showed that very low concentration of Bt protein detected in A.gossypii reared on Bt cotton.The transgenic Bt cotton SGK321 had no significant impacts on mortality,reproduction and life-table parameters of A.gossypii.The contents of carbohydrate,lipid and protein in A.gossypii,fed on transgenic Bt cotton for a long period of time,were also not significantly affected compared to fed on control cotton.Datum indicated that transgenic Bt cotton SGK321 had no direct acute toxic effects on A.gossypii in the long term.

关 键 词：转BT基因棉 非靶标害虫 棉蚜 代谢物质 ELISA 

分 类 号：S562.01[农业科学—作物学]