基因枪转化光温敏雄性不育小麦受体的选择与培养条件优化  

The Choice of Photo-thermo-sensitive Male Sterile Wheat Receptor Through Partical Bombardment and the Optimization of Culture Conditions

作　　者：王灵云[1] 张立平[1,2] 赵昌平[1,2] 张风廷[1,2] 樊建青[1] 苑少华 郭玉平 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]北京杂交小麦工程技术研究中心,北京100097

出　　处：《华北农学报》2011年第4期126-129,共4页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金项目(5091001);国家高技术研究与发展计划"863"项目(2009AA101102;2011AA10A106);农业部"948"项目(2009-Z4);北京市农业育种基础研究创新平台项目(D08070500690801;D111100001311002)

摘　　要：为了选择适宜的基因枪转化光温敏雄性不育小麦的受体,并对幼胚组织培养条件进行优化,以小麦光温敏雄性不育系BS210、BS366以及常规品种京411的幼胚为材料,分别对碳源、除草剂浓度和基因型进行筛选。结果表明,在以蔗糖和麦芽糖为碳源的诱导培养基上,幼胚的出愈率差异不显著;在以蔗糖和麦芽糖为碳源的分化培养基上,愈伤组织的分化率差异显著,其中添加麦芽糖的培养基上分化率较高;在对轰击过bar基因的幼胚愈伤组织进行筛选时,除草剂Biolaphos的最适浓度为2 mg/L。3个受体材料离体培养过程中出苗率差异显著,其中BS210出苗率高,是较优良的转化受体,为今后利用转基因技术改良光温敏雄性不育小麦奠定了基础。In order to choose the appropriate receptor of photo-thermo-sensitive male sterile wheat for bombardment,and optimize the Culture condition of immature embryos,We used immature embryos of 3 wheat lines,including photo-thermo-sensitive male sterile line BS210,BS366 and normal cultivar Jing 411,Carbon source,screening concentration and genotype were screened respectively.The study showed that the frequency of embryos callus formation is not significantly different between sucrose and maltose as the carbon source.While adding maltose in different mediums,the frequency of callus differentiation was higher than the medium adding sucrose.During screening callus that the bar gene had bombarded in,the optimal concentration of herbicides biolaphos was 2 mg/L.The seeding rate of three material was significantly different.The seeding rate of BS210 was higher than other plants,which is the best recipient for transformation.The research sets the foundation for the improvement of photo-thermo-sensitive male sterile wheat.

关 键 词：小麦幼胚 光温敏雄性不育 碳源 除草剂浓度 基因型 

分 类 号：S512.03[农业科学—作物学]