苏云金芽孢杆菌SHJ-5菌株杀虫晶体蛋白基因分析  

Identification of cry Gene from a Novel Bacillus thuringiensis Isolate SHJ-5

作　　者：杜立新[1] 董明[1] 王容燕[1] 马铭泽[2] 王金耀[1] 曹伟平[1] 宋健[1] 冯书亮[1] 

机构地区：[1]河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,河北保定071000 [2]河北省农林科学院,河北石家庄050051

出　　处：《华北农学报》2011年第4期194-197,共4页Acta Agriculturae Boreali-Sinica

基　　金：国家“863”计划项目(2011AA10A203);国家科技支撑项目(2006BAD08A02);国家科技支撑项目(2008BADA5B07)

摘　　要：苏云金芽孢杆菌SHJ-5菌株是从黑龙江采集的土样中分离获得的新菌株,对其伴孢晶体形状、基因型、蛋白表达以及杀虫活性进行了系统研究。菌株SHJ-5产生的伴孢晶体形状为钝菱形;利用PCR-RFLP技术对该菌株的基因型进行鉴定,结果表明,该菌株含有cry1Aa、cry1Ea、cry1Be、cry1Ah、cry1Ai、cry2Ab、cry1i和vip3A基因;SDS-PAGE晶体蛋白检测结果显示,该菌株表达130～150 kDa和60 kDa大小蛋白;生物活性测定表明,菌株SHJ-5对棉铃虫和小菜蛾有较高杀虫活性,校正死亡率分别为100%和96.7%。The novel Bacillus thuringiensis SHJ-5 was isolated from the soil in Heilongjiang province.This paper was a study on the strain SHJ-5,including that the shape of the crystal,the cry-type,protein expression and insecticidal activity.It produced blunt diamond-shaped crystals.It was identified that strain SHJ-5 contained cry1Aa,cry1Ea,cry1Be,cry1Ah,cry1Ai,cry2Ab,cry1I and vip3A gene by PCR-RFLP.SDS-PAGE analysis indicated that the strain produced a 130-150 kDa protein and a 60 kDa protein.Bioassay showed that strain SHJ-5 had high insecticidal activity to Helicoverpa armigera and Plutella xylostella.The corrected mortality to the two target insects was 100% and 96.7%,respectively.

关 键 词：苏云金芽孢杆菌 PCR-RFLP 克隆 杀虫活性 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]