山茛菪碱对大鼠移植肝脏保护作用的实验研究  

Experimental study on the preventive effects of anisodamine on donor liver in rats

作　　者：马凯[1] 卜献民[1] 戴显伟[1] 李延钧[2] 王亮[1] 戴阳[1] 

机构地区：[1]中国医科大学第二临床学院肝胆外科,沈阳110003 [2]抚顺矿务局总医院肿瘤科

出　　处：《中华器官移植杂志》2000年第2期86-88,共3页Chinese Journal of Organ Transplantation

基　　金：辽宁省自然科学基金!96 2 2 80

摘　　要：目的　利用山茛菪碱具有稳定细胞膜和抗氧自由基损伤的作用 ,在低温保存期间用山茛菪碱对移植肝脏进行保护 ,从而改善移植肝脏的质量。方法　建立大鼠下腔静脉内分流法自体原位肝移植动物模型。动态观察肝脏氧自由基及酶学的变化。结果　实验组用山茛菪碱可减少再灌流后脂质过氧化物酶 (LPO)的增加 ,超氧化物歧化酶 (SOD)和谷胱甘肽过氧化物酶 (GSH Px)的下降 ,丙氨酸转氨酶 (ALT)乳酸脱氢酶 (LDH)的升高 ,与对照组比较 ,P <0 .0 1。结论　再灌流前应用山茛菪碱能减少再灌流后氧自由基的产生 ,可减轻缺血—再灌流对移植肝脏的损伤。Objective To improve the quality of grafted liver, anisodamine was used to protect the grafted liver during the preservation of low temperature.Methods The model of orthotopic liver autotransplantation with intra inferior vana cava shunting method was set up. The changes of oxygen derived free radical and enzymes (LPO, SOD, GSH Px, ALT and LDH) were dynamically observed.Results Anisodamine could reduce the increase of LPO, ALT and LDH and the decrease of SOD and GSH Px significantly after reperfusion ( P < 0.01 ).Conclusions The use of anisodamine before reperfusion could reduce the production of oxygen derived free radical, therefore alleviate the injury of grafted liver caused by ischemia reperfusion.

关 键 词：肝移植 氧自由基 山茛菪碱 肝脏保护 实验研究 

分 类 号：R657.3[医药卫生—外科学] R975.5[医药卫生—临床医学]