人-鼠抗CD4嵌合抗体的生物学效应研究  

The biological effect of anti-CD4 human mouse chimeric antibody

作　　者：朱慧芬[1] 杨道锋[2] 王晓林[1] 朱志刚[1] 张悦[1] 沈关心[1] 

机构地区：[1]同济医科大学免疫学教研室,武汉430030 [2]同济医科大学附属同济医院传染科

出　　处：《中华器官移植杂志》2000年第2期92-94,共3页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金!393870 6 2 8

摘　　要：目的　探讨人 鼠抗CD4嵌合抗体的生物学效应。方法　观察CD4人 鼠嵌合抗体和鼠源性单抗对CD3、植物血凝素 (PHA)、白细胞介素 2 (IL 2 )及同种异体细胞诱导增殖的影响 ,以及其介导的抗体依赖性细胞介导的细胞毒性 (ADCC)作用。结果　提示CD4嵌合抗体和鼠源性CD4抗体对以上几种诱导剂诱导的增殖均有抑制作用 ,且抗体剂量越大 ,抑制作用越强。结论　CD4嵌合抗体和鼠源性抗体具有有效的免疫抑制作用。Objective To investigate the biological effect of anti CD4 human mouse chimeric antibody. Methods The effects of anti CD4 human mouse chimeric and mouse derived monoclonal antibody on CD3, PHA, IL 2, proliferation induced by allogenic cells and the propagation of ADCC mediated by these antibodies were observed. Results The propagation of PBMC induced by CD3, PHA, IL 2 and allogenic cells was inhibited by both the chimeric antibody and mouse monoclonal antibody with the inhibitory activity being directly proportional to the dosage of the antibodies. With a given dosage, the inhibitory activity induced by the chimeric antibody was higher than that by the mouse antibody. Conclusion The killing action of PBMC to cultured tumor cells could be promoted by both antibodies.

关 键 词：CD4嵌合抗体 增殖抑制 生物学效应 移植免疫学 

分 类 号：R392[医药卫生—免疫学]