牛血红蛋白用于兔离体心脏延时保存的研究  

Preservation of isolated rabbit hearts after normothermia storage with stroma free bovine hemoglobin solution

作　　者：康云帆[1] 蔡振杰[1] 胡军[1] 

机构地区：[1]第四军医大学西京医院心脏外科中心

出　　处：《中华器官移植杂志》2000年第2期95-97,共3页Chinese Journal of Organ Transplantation

摘　　要：目的　研究牛血红蛋白对兔离体心脏保存时限的作用。方法　在StThomasⅡ液中加入无机质游离牛血红蛋白 (SFBHB) ,用该液常温灌注离体兔心 6h作为实验组 ,用单纯StThomasⅡ液低温保存离体兔心作为对照组。每组大白兔均为 12只。结果　左室发展压 (LVDP)、冠状动脉流量恢复率 (CFR) ,实验组均显著高于对照组 ,P <0 .0 0 1;兔心保存 4h后 ,心肌组织ATP含量两组差异显著 (P <0 .0 5 ) ;心脏复跳 30min后 ,心肌组织内的丙二醛 (MDA)含量及冠状静脉窦引流液中的心肌磷酸激酶 (CPK)、乳酸脱氢酶 (LDH)的含量实验组明显低于对照组 ,两组比较差异有极显著性 (P <0 .0 1)。结论　在StThomasⅡ液中加入SFBHB ,可以显著提高离体兔心的保存效果。Objective To evaluate the protective effects of stroma free bovine hemoglobin (SFBHB) on preservation of the donor heart.Methods 24 isolated perfused rabbit hearts were equally divided into two groups. In control group, the cardiac arrest were induced with St Thomas Ⅱ solution and repeated every 30 min, the myocardial temperature remained at 40℃. In experimental group, the isolated rabbit hearts were perfused continuously with St Thomas Ⅱ solution containing stroma free bovine hemoglobin and the myocardial temperature remained at 37℃. The preservation lasted 6 h.Results There were significant differences between the two groups in the follows: (1) The mean values of the left ventricular developed pressure (LVDP) and coronary flow rate (CFR) were higher in experimental group ( P < 0.001 ); (2) The ATP level and myocardial oxygen uptake were significantly decreased in control group ( P < 0.001 ); (3) The mean values of released creatine phosphate kinase (CPK), lactec dehydrogenase (LDH) and myocardial contents of malondialdehyde (MDA) were higher in control group ( P < 0.01 ); (4) More water was present in the myocardial in control group ( P < 0.01 ); (5) The myocardial ultrastructural injuries were greater in control group.Conclusion The stroma free bovine hemoglobin could offer great protective effects on preservation of the isolated rabbit hearts.

关 键 词：牛血红蛋白 保存液 心脏移植 心脏保存 

分 类 号：R654.2[医药卫生—外科学]