猪下丘脑CART完整CDS区结构域分析  

The cDNA Cloning and Sequence Analysis of Fragment of CART mRNA in Porcine Hypothalamus

作　　者：李鹏飞[1] 李富禄[2] 于秀菊[2] 吕丽华 

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《山西农业大学学报（自然科学版）》2011年第5期469-473,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：农业部项目(2010-Z43);山西省留学基金和山西省引进人才专项基金项目(2008080);山西省国际科技合作项目(2010081002)

摘　　要：为获得猪可卡因苯异丙胺调控转录肽(Cocain and amphetamine-regulated transcript,CART)的完整CDS区并分析其结构域,根据GenBank中公布的其他物种CART的cDNA序列,设计了三对特异性引物,采用RT-PCR技术扩增出猪下丘脑组织CART mRNA的部分序列。经NCBI比对,与其他物种的相似性较高,提示该序列为猪CART的mRNA序列,CART mRNA在猪下丘脑上有表达;对该序列进行3D结构分析,发现猪CART蛋白在第40～116位氨基酸间存在一个CART超家族(CART Superfamily),这个超家族可能通过作为铁离子的配合基,在动物体内实现多种生理功能。In order to obtain and analysis the complete CDS and its domain of porcine CART （Coeain and amphetamine-- regulated transcript, CART）, based on the eDNA sequences of CART in other species published in GenBank, three pairs of specific primers were designed and part mRNA sequence was amplified by RT-PCR. By alignment with other species, the sequences had high similarities, suggesting that the sequence was the porcine CART,and the mRNA expressed in porcine hypothalamus; Analysis of the 3D structure of the CART protein,showed that a CART superfamily （CART Superfamily） existed between amino acids 40 to 116. The superfamily may be as the ligand of iron to achieve a variety of physiLological functions in vivo.

关 键 词：猪 CART 克隆 序列分析 

分 类 号：S828[农业科学—畜牧学]