甲基对硫磷人工抗原的合成及鉴定  

Synthesis and identification of methylparathion artificial antigen

作　　者：王刚垛[1] 马兆扬[1] 鱼涛[1] 何凤生[1] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所,北京100050

出　　处：《卫生研究》2000年第2期69-70,共2页Journal of Hygiene Research

基　　金：国家"九五"攻关基金! ( No.96 -90 6 -0 4-11)

摘　　要：为了合成甲基对硫磷(M1605)人工抗原,用醋酸-锌粉-盐酸还原甲基对硫磷,制备氨基甲基对硫磷,重氮化法使氨基甲基对硫磷与牛血清白蛋白(BSA)及中国鲎血蓝蛋白(TTH)偶合,合成人工抗原M1605-BSA、M1605-TTH。M1605-BSA免疫新西兰兔10周后,双向琼脂扩散试验和间接ELISA检测,证明得到了高价且具有较好特异性的多抗血清,成功地合成了甲基对硫磷人工抗原,为其免疫分析方法的建立提供了条件。In order to synthesize the artificial antigen methylparathion(M1605),methylparathion was reduced into amino methylparathion by using acetic acid zinc powder hydrochloric acid.Artificial antigens (M1605 BSA,M1605 TTH) were synthesized by conjugating amino methylparathion to bovine serum albumin(BSA) and tachypleus tridentatus hemocyanin (TTH) directly after diazotization.Rabbits were immunized with M1605 BSA for 10 weeks,and the high titer and high specificity antiserum from those rabbits was testified by double agar gel diffusion and indirect ELISA.The results showed that an artificial antigen was obtained successfully and this made it possible to establish the immunoassay of M1605.

关 键 词：甲基对硫磷 人工抗原 多抗血清 合成 鉴定 

分 类 号：R392.7[医药卫生—免疫学] R595.405[医药卫生—基础医学]