高温对大鼠红细胞免疫功能和胚胎发育的影响  

Effect of hyperthermia on the red-cell immune function of rats and its teratogenicity on developing embryos

作　　者：朱新安[1] 李勇[2] 

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830000 [2]北京医科大学卫生部生育健康研究重点实验室

出　　处：《卫生研究》2000年第2期89-90,共2页Journal of Hygiene Research

基　　金：国家自然科学基金! ( No.3 9870 6 97;3 9870 845 )

摘　　要：为研究高温(40℃)对孕10天龄大鼠红细胞免疫功能和胚胎生长发育的影响并探讨其作用机理,对孕鼠进行不同时间的热处理(0、0.5、1.0、2.5和5.0h)后,取静脉血检测红细胞C3b受体活性(花环)、红细胞免疫复合物和血清中循环免疫复合物含量;并用尼罗兰活体染色、光镜、电镜、组织化学等方法探讨高温对胚胎的致畸性。结果显示,高温使红细胞C3b受体花环、红细胞免疫复合物和循环免疫复合物水平升高,室温(22±2)℃恢复20h后,基本降至正常水平;随着高温处理时间的延长,畸胎率由0.9%(1/117)上升到18.3%(22/120),呈明显时间-效应关系,活性染色发现在胚胎肢芽、颅面部和腮弓等部位出现死亡细胞,镜检发现高温能损害细胞的超微结构和诱发胚胎细胞凋亡过度。提示:热应激时,母体红细胞免疫功能的增强可能是高温致畸作用的机制之一。After rats on gestation day 10 exposed to hyperthermia(40℃) for 0 5,1 0,2 5and5 0h,redcell C3b receptor activity(aggregate),red cell immune complex,circulating immune complex,Nile blue sulfate(NBS) vital staining,electronic microscope and histochemical techniques were used in investigate whether the hyperthermia was disturb red cell immune functions of pregnant rats and played some role in teratogenesis.The results showed that hyperthermia increased the level of red cell C3b receptor activity,red cell immune complex and circulating immune complex.On the other hand,many of them also decreased to normal level after rats were placed in room temperature for 20h.Following the extension of hyperthermic exposure,the teratogenic incidence increased from 0 9%(1/117)to 18 3%(22/120),in apparent time effect relationship,when pregnant rats of gestation day 11 5 were killed.It was found that hyperthermia induced over apoptosis of embryos in many areas,and damaged cellular ultrastructures by NBS staining and electronic microscope.These findings show that there may be some relationship between red cell immune function of pregnant rats and developmental defects of embryos after exposure to hyperthermia.

关 键 词：高温 红细胞免疫功能 胚胎发育 致畸性 畸胎率 

分 类 号：R594.1[医药卫生—内科学] R321.6[医药卫生—临床医学]