锌缺乏对培养小鼠胚胎长骨骺板c-fos基因转录和表达的影响  

Effects of zinc deficiency on the c-fos gene expression and transcription in the epiphyses of fetal mouse long bone in culture

作　　者：马丽英[1] 杨青[1] 刘开泰[2] 李灵芝 张永亮[3] 李云[1] 王瑞淑[1] 

机构地区：[1]华西医科大学公共卫生学院,成都600044 [2]新疆医科大学 [3]中国人民解放军天津市武警医学院

出　　处：《卫生研究》2000年第2期106-108,共3页Journal of Hygiene Research

基　　金：国家自然科学基金!( No.3 96 0 0 12 2)

摘　　要：采用体外培养方法研究锌缺乏对小鼠胚胎长骨骺板c-fos基因(c-fos)转录与表达的影响。将孕龄16天的小鼠胚胎长骨体外培养48小时(培养基为GBJb),通过免疫组化及原位杂交方法观察培养长骨骺板c-fos转录与表达的变化,并用图像分析系统对结果进行分析。根据培养基的锌浓度将实验分为对照组、缺锌组、缺锌补锌组、锌刺激量组。结果显示当缺锌时,c-fos的蛋白和mRNA表达均减弱,阳性细胞数与对照组相比明显降低(P<0.05);当培养基锌浓度为100μmol/L时,小鼠胚胎长骨骺板增殖区、肥大区和静止区c-fos转录与表达都有不同程度的增加。研究表明锌可影响c-fos转录与表达。The effects of zinc on c fos gene expression and transcription in the epiphyses of fetal long bone were studied in mice.The long bones of 16 day fetal mouse were cultured for 48 hours(in medium GBJb) and then used for measuring c fos gene expression and transcription by immunnohistochemistry and in situ hybridization.The result was analyzed by an imaged analyses system.The experiment was divided into zinc control group(ZC),zinc deficiency group(ZD),zinc deficiency replenish group(ZDR) and zinc stimulatory group(ZS) respectively.The result showed that 1)zinc deficiency caused c fos protein and mRNA expression and the number of reactive cell decreased. 2)when zinc concentration of medium was 100μmol/L,the expression and transcription of c fos gene were increased in hypertrophic,proliferative and resting zone of epiphyses.The research suggested that zinc could affect the expression of c fos gene in mouse fetal long bone.

关 键 词：锌缺乏 胚胎长骨骺板 C-FOS 基因转录 基因表达 

分 类 号：R591.1[医药卫生—内科学] Q132.7[医药卫生—临床医学]