凋亡相关蛋白Fas及Caspase-3在多囊肝病中的表达及临床意义  

Expression of Apoptosis-Associated Protein Fas and Caspase-3 in Patients with Polycystic Liver Disease

作　　者：米富海[1] 李连生[1] 李英[1] 

机构地区：[1]青海红十字医院,810000

出　　处：《青海医药杂志》2011年第12期1-3,共3页Qinghai Medical Journal

摘　　要：目的:观察凋亡诱导蛋白(Fas)及凋亡执行蛋白(Caspase-3)在多囊肝病中的表达及临床意义。方法:对43例多囊肝病患者囊壁肝组织和周围正常肝组织分别采用TUNEL观察标本细胞凋亡,免疫组织化学方法检测凋亡诱导蛋白Fas,凋亡抑制蛋白Bcl-2,凋亡执行蛋白Caspase-3表达并结合临床资料进行分析。结果:TUNEL显示囊肿壁肝细胞的凋亡指数(AI)明显高于正常组织;Fas和Caspase-3在囊肿壁肝细胞中的表达明显高于周围正常组织;Bcl-2在囊肿壁肝细胞中的表达明显低于周围正常组织。相关性分析表明,囊肿壁肝细胞AI与Fas和Caspase-3的表达呈正相关;与Bcl-2的表达呈负相关。临床相关分析表明,TUNEL表达阳性与否和年龄、性别、家族史、合并肝外囊肿、症状、血清白蛋白和谷丙转氨酶(GPT)水平无关;Fas的表达与合并肝外囊肿有关,但与年龄、性别、家族史、症状、血清白蛋白和GPT水平无关。结论:肝细胞凋亡在多囊肝病的发生发展中起重要作用。Fas的表达对于判断多囊肝病人是否合并肝外囊肿具有重要的指导意义。Objective： To assess clinical significance Of hepatocellular apoptosis in polycystic liver disease （PCLD）. Methods ： Cell samples of cyst wall and normal liver were collected, and the cell apoptosis was observed using TUNEL in 42; patients with PCLD. The expression of apoptosis protein Fas, Caspase - 3 and Bcl - 2 were detected by immunohistochemistry. Results ：TUNEL staining disclosed apoptosis index （AI） in cyst was significantly higher than in normal liver tissue. The expression of Fas and Caspase -3 in cyst wall cell was significantly higher than in normal live：r tissue, contrary to Bcl -2. There no was a relationship among Tunel expression and age, gender, family history, symptom, albumin and GPT level; but the expression of Fas was positively correlated to AI in cyst. The expression of Fas associated with extrahepatie cyst but no relation to age, gender, family history, syruptom, albumin and GPT level. Conclusions： Hepatocellular PCLD. Expression of Fas is a important to indicate whether apoptosis plays an important role in pathogenesis of patients with PCLD have extrahepatic cyst.

关 键 词：多囊肝病 凋亡诱导蛋白(Fas) 凋亡抑制蛋白(Bcl-2) 凋亡执行蛋白(Caspase-3) 

分 类 号：R657.3[医药卫生—外科学]