ARMS-PCR在水稻米香基因Fgr检测中的应用  

The Application of Detection Fragrance Rice Fgr with Tetra Primer Amplification Refractory Mutation System PCR

作　　者：陈晓军[1] 王敬东[1] 殷延勃[1] 马洪文[1] 宋玉霞[1] 

机构地区：[1]宁夏农业生物技术重点实验室,宁夏银川750002

出　　处：《华北农学报》2011年第B12期1-4,共4页Acta Agriculturae Boreali-Sinica

基　　金：宁夏回族自治区自然科学基金(NZ0974);宁夏回族自治区科技攻关项目(KGZ-16-07-02)

摘　　要：根据米香基因的测序结果,设计了4对引物,在0.8%琼脂糖上电泳检测Fgr基因。结果表明,该方法较好地检测粳稻和籼稻米香基因(Fgr),为大规模进行分子标记辅助育种提供有效手段。Fgr gene is a major effect gene that controls fragrance trait in rice. The aim of this study was to establish a tetra primer ARMS PCR method for the detection of Fgr. According the sequence of the fragrance rice material,were four primer designed for detection fragrance rice Fgr in 0.8% agarose electrophoresis. The result showed that the method was efficient in detection Fgr and provided the better method for MAS.

关 键 词：ARMSPCR FGR SNP 香稻 

分 类 号：Q78[生物学—分子生物学]