河北省小麦孢囊线虫种类鉴定  

Identification of Cereal Cyst Nematode Isolated from Hebei Province

作　　者：马娟[1] 李秀花[1] 于海滨[1] 陈书龙[1] 

机构地区：[1]河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心,河北保定071000

出　　处：《华北农学报》2011年第B12期168-173,共6页Acta Agriculturae Boreali-Sinica

基　　金：公益性行业科研专项(200903040);科技部国际合作项目(2009DFB30230)

摘　　要：近年来小麦孢囊线虫病在河北省发生十分普遍,给小麦生产造成了严重损失。可以侵染小麦的孢囊线虫种类很多,对于病原的准确鉴定是防治病害的基础。本试验从形态学和分子生物学两方面对从河北省不同地区采集到的37个小麦孢囊线虫群体进行了比较分析和鉴定。经过对线虫ITS-rDNA序列分析,发现采集到的线虫ITS区序列差异很小,为同一种类,其ITS-rDNA序列与燕麦孢囊线虫Heterodera avenae Wollenweber,1924的ITS-rDNA序列同源性高于99%。通过光学显微镜进行形态学测量和观察,其主要形态学特征与燕麦孢囊线虫H.avenae的相近。综合其形态与分子序列特征,确定河北省小麦孢囊线虫病的病原线虫为H.avenae。The cereal cyst nematode disease caused serious damage to the production of wheat in recent years. Accurate diagnosis is the basis for controlling this disease. 37 cereal cyst nematode populations were extracted from different areas of Hebei province in this study. The morphological and molecular characters of the nematodes were systemically studied. The difference of ITS-rDNA sequences of all the tested nematode was small and these sequences are less than 1% differences between local populations and tteterodera avenae. The main morphological and morphometrical characteristics of different populations were studied. They were mostly close to H. avenae in morphology. We confirmed that the nematode populations that extracted from Hebei province are Heterodera avenae based on morphological, morphometrical and molecular characters.

关 键 词：小麦孢囊线虫 形态学 分子生物学 ITS-RDNA 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]