甜叶菊的组织培养  

Plant Tissue Culture of Stevia rebaudiana Bertoni

作　　者：崔广荣[1,2] 何克勤[1,2] 胡能兵[1,2] 林平[1,2] 张子学[1,2] 王其斌 简善亮 

机构地区：[1]安徽科技学院植物科学学院,安徽凤阳233100 [2]安徽省甜叶菊工程技术研究中心,安徽蚌埠233000

出　　处：《安徽科技学院学报》2011年第5期23-28,共6页Journal of Anhui Science and Technology University

基　　金：安徽省教育厅省级重点自然科学基金项目(KJ2010A073);蚌埠市科技计划项目(科字051)

摘　　要：以甜叶菊茎段为外植体、MS为基本培养基,研究了消毒剂70%酒精和0.1%升汞的不同时间处理组合的灭菌效果和对试管苗再生的影响,同时还探讨了不同种类、不同浓度植物生长调节剂对试管苗增殖、生根及移栽的影响。结果表明7,0%酒精处理15～30s+0.1%升汞处理4m较为适合茎段外植体灭菌,污染率低、苗再生率高;6-BA与NAA组合的试管苗增殖系数比KT、Ad与NAA组合要高,培养基中添加1.0mg/L 6-BA+0.1～0.2mg/L NAA植物生长调节剂组合适合试管苗增殖,增殖系数可达7.4;培养基中添加0.0～0.5mg/L的IBA或NAA均适合试管苗生根,生根的试管苗移栽成活率较高。The stem -sections were chosen as explants for plant tissue culture in the MS basic medium. The test investigated the influences of disinfectors 70% alcohol and 0.1% HgC12 on sterilizing effects and shoot regenera- tion at first. And then, this test studied the effects of the plant growth regulators on shoot proliferating, rooting and transplanting too. The results showed that 70% alcohol treated for 15 - 30s with 0.1% HgCI2 treated for 4m was suitable for explants sterilizing and got lower explants infectious ratio and higher shoot formation ratio. The combinations of 6 - BA and NAA got higher proliferation coefficient than the combinations of Ad and NAA or the combinations of KT and NAA. The basic MS medium with 1.0mg/L 6 - BA and 0.1 - 0.2mg/L NAA was suit- able for shoot proliferation, and the coefficient of proliferation could reach 7.4. The 1/2 MS medium with 0.0 - 0.5mg/L IBA or NAA was suitable for shoot rooting and the survival rate was high.

关 键 词：甜叶菊 组织培养 植物生长调节剂 

分 类 号：S566.9[农业科学—作物学]